Fruit and vegetable intake assessed by food frequency questionnaire and plasma carotenoids

A validation study in adults

Tracy L. Burrows, Melinda J. Hutchesson, Megan E. Rollo, May Boggess, Maya Guest, Clare E. Collins

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dietary validation studies of self-reported fruit and vegetable intake should ideally include measurement of plasma biomarkers of intake. The aim was to conduct a validation study of self-reported fruit and vegetable intakes in adults, using the Australian Eating Survey (AES) food frequency questionnaire (FFQ), against a range of plasma carotenoids. Dietary intakes were assessed using the semi-quantitative 120 item AES FFQ. Fasting plasma carotenoids (α- and β-carotene, lutein/zeaxanthin, lycopene and cryptoxanthin) were assessed using high performance liquid chromatography in a sample of 38 adult volunteers (66% female). Significant positive correlations were found between FFQ and plasma carotenoids for α-carotene, β-carotene and lutein/zeaxanthin (52%, 47%, 26%, p < 0.001, 0.003, 0.041; respectively) and relationships between plasma carotenoids (except lycopene) and weight status metrics (BMI, waist circumference, fat mass) were negative and highly significant. The results of the current study demonstrate that carotenoid intakes as assessed by the AES FFQ are significantly related to plasma concentrations of α-carotene, β-carotene and lutein/zeaxanthin, the carotenoids commonly found in fruit and vegetables. Lower levels of all plasma carotenoids, except lycopene, were found in individuals with higher BMI. We conclude that the AES can be used to measure fruit and vegetable intakes with confidence.

Original languageEnglish (US)
Pages (from-to)3240-3251
Number of pages12
JournalNutrients
Volume7
Issue number5
DOIs
StatePublished - May 6 2015

Fingerprint

Validation Studies
vegetable consumption
food frequency questionnaires
fruit consumption
Carotenoids
Vegetables
Fruit
carotenoids
Eating
carotenes
zeaxanthin
lycopene
lutein
ingestion
Lutein
Food
waist circumference
Surveys and Questionnaires
volunteers
fasting

Keywords

  • Diet assessment validation
  • Food frequency questionnaire
  • Fruit vegetable intake
  • Plasma carotenoids

ASJC Scopus subject areas

  • Food Science

Cite this

Fruit and vegetable intake assessed by food frequency questionnaire and plasma carotenoids : A validation study in adults. / Burrows, Tracy L.; Hutchesson, Melinda J.; Rollo, Megan E.; Boggess, May; Guest, Maya; Collins, Clare E.

In: Nutrients, Vol. 7, No. 5, 06.05.2015, p. 3240-3251.

Research output: Contribution to journalArticle

Burrows, Tracy L. ; Hutchesson, Melinda J. ; Rollo, Megan E. ; Boggess, May ; Guest, Maya ; Collins, Clare E. / Fruit and vegetable intake assessed by food frequency questionnaire and plasma carotenoids : A validation study in adults. In: Nutrients. 2015 ; Vol. 7, No. 5. pp. 3240-3251.
@article{7f6c71000b84488c951337ab311bf30a,
title = "Fruit and vegetable intake assessed by food frequency questionnaire and plasma carotenoids: A validation study in adults",
abstract = "Dietary validation studies of self-reported fruit and vegetable intake should ideally include measurement of plasma biomarkers of intake. The aim was to conduct a validation study of self-reported fruit and vegetable intakes in adults, using the Australian Eating Survey (AES) food frequency questionnaire (FFQ), against a range of plasma carotenoids. Dietary intakes were assessed using the semi-quantitative 120 item AES FFQ. Fasting plasma carotenoids (α- and β-carotene, lutein/zeaxanthin, lycopene and cryptoxanthin) were assessed using high performance liquid chromatography in a sample of 38 adult volunteers (66{\%} female). Significant positive correlations were found between FFQ and plasma carotenoids for α-carotene, β-carotene and lutein/zeaxanthin (52{\%}, 47{\%}, 26{\%}, p < 0.001, 0.003, 0.041; respectively) and relationships between plasma carotenoids (except lycopene) and weight status metrics (BMI, waist circumference, fat mass) were negative and highly significant. The results of the current study demonstrate that carotenoid intakes as assessed by the AES FFQ are significantly related to plasma concentrations of α-carotene, β-carotene and lutein/zeaxanthin, the carotenoids commonly found in fruit and vegetables. Lower levels of all plasma carotenoids, except lycopene, were found in individuals with higher BMI. We conclude that the AES can be used to measure fruit and vegetable intakes with confidence.",
keywords = "Diet assessment validation, Food frequency questionnaire, Fruit vegetable intake, Plasma carotenoids",
author = "Burrows, {Tracy L.} and Hutchesson, {Melinda J.} and Rollo, {Megan E.} and May Boggess and Maya Guest and Collins, {Clare E.}",
year = "2015",
month = "5",
day = "6",
doi = "10.3390/nu7053240",
language = "English (US)",
volume = "7",
pages = "3240--3251",
journal = "Nutrients",
issn = "2072-6643",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

TY - JOUR

T1 - Fruit and vegetable intake assessed by food frequency questionnaire and plasma carotenoids

T2 - A validation study in adults

AU - Burrows, Tracy L.

AU - Hutchesson, Melinda J.

AU - Rollo, Megan E.

AU - Boggess, May

AU - Guest, Maya

AU - Collins, Clare E.

PY - 2015/5/6

Y1 - 2015/5/6

N2 - Dietary validation studies of self-reported fruit and vegetable intake should ideally include measurement of plasma biomarkers of intake. The aim was to conduct a validation study of self-reported fruit and vegetable intakes in adults, using the Australian Eating Survey (AES) food frequency questionnaire (FFQ), against a range of plasma carotenoids. Dietary intakes were assessed using the semi-quantitative 120 item AES FFQ. Fasting plasma carotenoids (α- and β-carotene, lutein/zeaxanthin, lycopene and cryptoxanthin) were assessed using high performance liquid chromatography in a sample of 38 adult volunteers (66% female). Significant positive correlations were found between FFQ and plasma carotenoids for α-carotene, β-carotene and lutein/zeaxanthin (52%, 47%, 26%, p < 0.001, 0.003, 0.041; respectively) and relationships between plasma carotenoids (except lycopene) and weight status metrics (BMI, waist circumference, fat mass) were negative and highly significant. The results of the current study demonstrate that carotenoid intakes as assessed by the AES FFQ are significantly related to plasma concentrations of α-carotene, β-carotene and lutein/zeaxanthin, the carotenoids commonly found in fruit and vegetables. Lower levels of all plasma carotenoids, except lycopene, were found in individuals with higher BMI. We conclude that the AES can be used to measure fruit and vegetable intakes with confidence.

AB - Dietary validation studies of self-reported fruit and vegetable intake should ideally include measurement of plasma biomarkers of intake. The aim was to conduct a validation study of self-reported fruit and vegetable intakes in adults, using the Australian Eating Survey (AES) food frequency questionnaire (FFQ), against a range of plasma carotenoids. Dietary intakes were assessed using the semi-quantitative 120 item AES FFQ. Fasting plasma carotenoids (α- and β-carotene, lutein/zeaxanthin, lycopene and cryptoxanthin) were assessed using high performance liquid chromatography in a sample of 38 adult volunteers (66% female). Significant positive correlations were found between FFQ and plasma carotenoids for α-carotene, β-carotene and lutein/zeaxanthin (52%, 47%, 26%, p < 0.001, 0.003, 0.041; respectively) and relationships between plasma carotenoids (except lycopene) and weight status metrics (BMI, waist circumference, fat mass) were negative and highly significant. The results of the current study demonstrate that carotenoid intakes as assessed by the AES FFQ are significantly related to plasma concentrations of α-carotene, β-carotene and lutein/zeaxanthin, the carotenoids commonly found in fruit and vegetables. Lower levels of all plasma carotenoids, except lycopene, were found in individuals with higher BMI. We conclude that the AES can be used to measure fruit and vegetable intakes with confidence.

KW - Diet assessment validation

KW - Food frequency questionnaire

KW - Fruit vegetable intake

KW - Plasma carotenoids

UR - http://www.scopus.com/inward/record.url?scp=84929346561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929346561&partnerID=8YFLogxK

U2 - 10.3390/nu7053240

DO - 10.3390/nu7053240

M3 - Article

VL - 7

SP - 3240

EP - 3251

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 5

ER -