Fractionation of Marek's disease virus-induced lymphoma by velocity sedimentation and association of infectivity with cellular fractions with and without tumor antigen expression.

J. M. Sharma

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Cell suspensions of lymphomas induced by Marek's disease (MD) virus were fractionated by sedimentation at unit gravity on a continuous gradient of bovine fetal serum. Cells in various fractions were examined for MD tumor-associated surface antigen (MATSA) by indirect immunofluorescence, using specific antibody, and for viral infectivity by cocultivating fractionated cells with permissive monolayer cells of duck embryo fibroblasts. Most MATSA-bearing cells in the lymphomas sedimented at a sedimentation velocity of greater than 3.0 mm/hour, whereas smaller, slow-sedimentating cells were generally devoid of MATSA expression. Viral infectivity was associated with MATSA-bearing and MATSA-lacking fractions. Within the limits of the experimental procedures used, this observation provided evidence that presence of MD virus genome in lymphocytes doses not result in concurrent expression of detectable MATSA and that MATSA likely represents another stage of interaction between MD virus and certain lymphocytes.

Original languageEnglish (US)
Pages (from-to)483-486
Number of pages4
JournalAmerican journal of veterinary research
Volume42
Issue number3
StatePublished - Mar 1 1981

ASJC Scopus subject areas

  • veterinary(all)

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