Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information

Andrea L. Himmelberger; Theresa F. Spear; Jessica A. Satkoski; Debra A. George; Wendy T. Garnica; Venkat S. Malladi; David G. Smith; Kristen M. Webb; Marc W. Allard; Sreetharan Kanthaswamy

doi:10.1111/j.1556-4029.2007.00615.x

Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information

Andrea L. Himmelberger, Theresa F. Spear, Jessica A. Satkoski, Debra A. George, Wendy T. Garnica, Venkat S. Malladi, David G. Smith, Kristen M. Webb, Marc W. Allard, Sreetharan Kanthaswamy

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The 608-bp hypervariable region 1 (HV1) sequences from 36 local dogs were analyzed to characterize the population genetic structure of canid mitochondrial DNA (mtDNA). Sixteen haplotypes were identified. A 417-bp segment of this sequence was compared with GenBank sequences from a geographically representative sample of 201 dogs, two coyotes, and two wolves. Sixty-six haplotypes were identified including 62 found only in domestic dogs. Fourteen of these correspond to the 16 local haplotypes and were among the most frequent haplotypes. The local sample was judged to be representative of the much broader geographic sample. No correlation was observed between local haplotypes and the owner's characterization of dog breed. A 60-bp variation "hotspot" within the canid HV1 was identified as a potentially valuable molecular tool, particularly for assaying limited or degraded DNA samples.

Original language	English (US)
Pages (from-to)	81-89
Number of pages	9
Journal	Journal of Forensic Sciences
Volume	53
Issue number	1
DOIs	https://doi.org/10.1111/j.1556-4029.2007.00615.x
State	Published - Jan 2008
Externally published	Yes

Keywords

Domestic dog
Forensic science
Geographic origin
Hypervariable region 1
Mitochondrial DNA
Mixed and pure breed studies
Sequence variation
Trace evidence

ASJC Scopus subject areas

Pathology and Forensic Medicine
Genetics

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10.1111/j.1556-4029.2007.00615.x

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Himmelberger, A. L., Spear, T. F., Satkoski, J. A., George, D. A., Garnica, W. T., Malladi, V. S., Smith, D. G., Webb, K. M., Allard, M. W., & Kanthaswamy, S. (2008). Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information. Journal of Forensic Sciences, 53(1), 81-89. https://doi.org/10.1111/j.1556-4029.2007.00615.x

Himmelberger, AL, Spear, TF, Satkoski, JA, George, DA, Garnica, WT, Malladi, VS, Smith, DG, Webb, KM, Allard, MW & Kanthaswamy, S 2008, 'Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information', Journal of Forensic Sciences, vol. 53, no. 1, pp. 81-89. https://doi.org/10.1111/j.1556-4029.2007.00615.x

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abstract = "The 608-bp hypervariable region 1 (HV1) sequences from 36 local dogs were analyzed to characterize the population genetic structure of canid mitochondrial DNA (mtDNA). Sixteen haplotypes were identified. A 417-bp segment of this sequence was compared with GenBank sequences from a geographically representative sample of 201 dogs, two coyotes, and two wolves. Sixty-six haplotypes were identified including 62 found only in domestic dogs. Fourteen of these correspond to the 16 local haplotypes and were among the most frequent haplotypes. The local sample was judged to be representative of the much broader geographic sample. No correlation was observed between local haplotypes and the owner's characterization of dog breed. A 60-bp variation {"}hotspot{"} within the canid HV1 was identified as a potentially valuable molecular tool, particularly for assaying limited or degraded DNA samples.",

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author = "Himmelberger, {Andrea L.} and Spear, {Theresa F.} and Satkoski, {Jessica A.} and George, {Debra A.} and Garnica, {Wendy T.} and Malladi, {Venkat S.} and Smith, {David G.} and Webb, {Kristen M.} and Allard, {Marc W.} and Sreetharan Kanthaswamy",

year = "2008",

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doi = "10.1111/j.1556-4029.2007.00615.x",

language = "English (US)",

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AU - Spear, Theresa F.

AU - Satkoski, Jessica A.

AU - George, Debra A.

AU - Garnica, Wendy T.

AU - Malladi, Venkat S.

AU - Smith, David G.

AU - Webb, Kristen M.

AU - Allard, Marc W.

AU - Kanthaswamy, Sreetharan

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KW - Domestic dog

KW - Forensic science

KW - Geographic origin

KW - Hypervariable region 1

KW - Mitochondrial DNA

KW - Mixed and pure breed studies

KW - Sequence variation

KW - Trace evidence

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Forensic utility of the mitochondrial hypervariable region 1 of domestic dogs, in conjunction with breed and geographic information

Abstract

Keywords

ASJC Scopus subject areas

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