Folding analytical devices for electrochemical ELISA in hydrophobic RH paper

Ana C. Glavan, Dionysios C. Christodouleas, Bobak Mosadegh, Hai Dong Yu, Barbara Smith, Joshua Lessing, M. Teresa Fernández-Abedul, George M. Whitesides

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL-1 (102 pmol L-1), a value within the range of clinically relevant concentrations. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)11999-12007
Number of pages9
JournalAnalytical Chemistry
Volume86
Issue number24
DOIs
StatePublished - Dec 16 2014
Externally publishedYes

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Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies
Enzymes
Point-of-Care Systems
Antigens
Plasmodium falciparum
Surface Antigens
Serum
Immobilization
Limit of Detection
Buffers
Electrodes
Immunoglobulin G
Rabbits
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry
  • Medicine(all)

Cite this

Glavan, A. C., Christodouleas, D. C., Mosadegh, B., Yu, H. D., Smith, B., Lessing, J., ... Whitesides, G. M. (2014). Folding analytical devices for electrochemical ELISA in hydrophobic RH paper. Analytical Chemistry, 86(24), 11999-12007. https://doi.org/10.1021/ac5020782

Folding analytical devices for electrochemical ELISA in hydrophobic RH paper. / Glavan, Ana C.; Christodouleas, Dionysios C.; Mosadegh, Bobak; Yu, Hai Dong; Smith, Barbara; Lessing, Joshua; Fernández-Abedul, M. Teresa; Whitesides, George M.

In: Analytical Chemistry, Vol. 86, No. 24, 16.12.2014, p. 11999-12007.

Research output: Contribution to journalArticle

Glavan, AC, Christodouleas, DC, Mosadegh, B, Yu, HD, Smith, B, Lessing, J, Fernández-Abedul, MT & Whitesides, GM 2014, 'Folding analytical devices for electrochemical ELISA in hydrophobic RH paper', Analytical Chemistry, vol. 86, no. 24, pp. 11999-12007. https://doi.org/10.1021/ac5020782
Glavan AC, Christodouleas DC, Mosadegh B, Yu HD, Smith B, Lessing J et al. Folding analytical devices for electrochemical ELISA in hydrophobic RH paper. Analytical Chemistry. 2014 Dec 16;86(24):11999-12007. https://doi.org/10.1021/ac5020782
Glavan, Ana C. ; Christodouleas, Dionysios C. ; Mosadegh, Bobak ; Yu, Hai Dong ; Smith, Barbara ; Lessing, Joshua ; Fernández-Abedul, M. Teresa ; Whitesides, George M. / Folding analytical devices for electrochemical ELISA in hydrophobic RH paper. In: Analytical Chemistry. 2014 ; Vol. 86, No. 24. pp. 11999-12007.
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