Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo

Alexander Christov; Erbin Dai; Liying Liu; Haiyan Guan; Mark A. Bernards; Paul B. Cavers; David Susko; Alexandra Lucas

doi:10.1117/12.465232

Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo

Alexander Christov, Erbin Dai, Liying Liu, Haiyan Guan, Mark A. Bernards, Paul B. Cavers, David Susko, Alexandra Lucas

Research output: Contribution to journal › Conference article › peer-review

Abstract

In order to assess the capacity for in vivo fluorescence spectroscopic analysis of arterial collagen and elastin, fluorescence emission intensity (FEI) was recorded from rabbit aorta after angioplasty and stent implant, and correlated with extracted elastin and collagen content. FEI from saline treated rabbits after stent implant was higher (p<0.05) between 485 and 500 nm than after anti-inflammatory treatment. FEI was significantly decreased (p<0.05) after implantation of shorter stents at 476-500 nm. Multiple regression analysis demonstrated an excellent correlation between FEI and elastin and HPLC-measured collagen content at 486-500 nm (adj. R² values > 0.931) and 476-480 nm (adj. R² values up to 0.950 for selected amino acids) respectively. Conclusions: FEI recorded in vivo from arterial intimal surface, can be successfully used for quantitative assessment of compositional changes in connective tissue. Stent implant can induce changes in intimal arterial structure at discrete sites distant from the stent implant site.

Original language	English (US)
Pages (from-to)	77-88
Number of pages	12
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4613
DOIs	https://doi.org/10.1117/12.465232
State	Published - 2002
Externally published	Yes
Event	Optical Biopsy IV - San Jose, CA, United States Duration: Jan 21 2002 → Jan 23 2002

Keywords

Arteries
Atherosclerosis
Collagen
Elastin
Fluorescence
Optical analysis
Spectroscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.465232

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Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo. / Christov, Alexander; Dai, Erbin; Liu, Liying et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4613, 2002, p. 77-88.

Research output: Contribution to journal › Conference article › peer-review

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title = "Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo",

abstract = "In order to assess the capacity for in vivo fluorescence spectroscopic analysis of arterial collagen and elastin, fluorescence emission intensity (FEI) was recorded from rabbit aorta after angioplasty and stent implant, and correlated with extracted elastin and collagen content. FEI from saline treated rabbits after stent implant was higher (p<0.05) between 485 and 500 nm than after anti-inflammatory treatment. FEI was significantly decreased (p<0.05) after implantation of shorter stents at 476-500 nm. Multiple regression analysis demonstrated an excellent correlation between FEI and elastin and HPLC-measured collagen content at 486-500 nm (adj. R2 values > 0.931) and 476-480 nm (adj. R2 values up to 0.950 for selected amino acids) respectively. Conclusions: FEI recorded in vivo from arterial intimal surface, can be successfully used for quantitative assessment of compositional changes in connective tissue. Stent implant can induce changes in intimal arterial structure at discrete sites distant from the stent implant site.",

keywords = "Arteries, Atherosclerosis, Collagen, Elastin, Fluorescence, Optical analysis, Spectroscopy",

author = "Alexander Christov and Erbin Dai and Liying Liu and Haiyan Guan and Bernards, {Mark A.} and Cavers, {Paul B.} and David Susko and Alexandra Lucas",

year = "2002",

doi = "10.1117/12.465232",

language = "English (US)",

volume = "4613",

pages = "77--88",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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note = "Optical Biopsy IV ; Conference date: 21-01-2002 Through 23-01-2002",

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T1 - Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo

AU - Christov, Alexander

AU - Dai, Erbin

AU - Liu, Liying

AU - Guan, Haiyan

AU - Bernards, Mark A.

AU - Cavers, Paul B.

AU - Susko, David

AU - Lucas, Alexandra

PY - 2002

Y1 - 2002

N2 - In order to assess the capacity for in vivo fluorescence spectroscopic analysis of arterial collagen and elastin, fluorescence emission intensity (FEI) was recorded from rabbit aorta after angioplasty and stent implant, and correlated with extracted elastin and collagen content. FEI from saline treated rabbits after stent implant was higher (p<0.05) between 485 and 500 nm than after anti-inflammatory treatment. FEI was significantly decreased (p<0.05) after implantation of shorter stents at 476-500 nm. Multiple regression analysis demonstrated an excellent correlation between FEI and elastin and HPLC-measured collagen content at 486-500 nm (adj. R2 values > 0.931) and 476-480 nm (adj. R2 values up to 0.950 for selected amino acids) respectively. Conclusions: FEI recorded in vivo from arterial intimal surface, can be successfully used for quantitative assessment of compositional changes in connective tissue. Stent implant can induce changes in intimal arterial structure at discrete sites distant from the stent implant site.

AB - In order to assess the capacity for in vivo fluorescence spectroscopic analysis of arterial collagen and elastin, fluorescence emission intensity (FEI) was recorded from rabbit aorta after angioplasty and stent implant, and correlated with extracted elastin and collagen content. FEI from saline treated rabbits after stent implant was higher (p<0.05) between 485 and 500 nm than after anti-inflammatory treatment. FEI was significantly decreased (p<0.05) after implantation of shorter stents at 476-500 nm. Multiple regression analysis demonstrated an excellent correlation between FEI and elastin and HPLC-measured collagen content at 486-500 nm (adj. R2 values > 0.931) and 476-480 nm (adj. R2 values up to 0.950 for selected amino acids) respectively. Conclusions: FEI recorded in vivo from arterial intimal surface, can be successfully used for quantitative assessment of compositional changes in connective tissue. Stent implant can induce changes in intimal arterial structure at discrete sites distant from the stent implant site.

KW - Arteries

KW - Atherosclerosis

KW - Collagen

KW - Elastin

KW - Fluorescence

KW - Optical analysis

KW - Spectroscopy

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DO - 10.1117/12.465232

M3 - Conference article

AN - SCOPUS:0036049868

SN - 0277-786X

VL - 4613

SP - 77

EP - 88

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Biopsy IV

Y2 - 21 January 2002 through 23 January 2002

ER -

Fluorescence spectroscopic analysis (FSA) detects quantitative changes in atherosclerotic plaque collagen and elastin content in vivo

Abstract

Keywords

ASJC Scopus subject areas

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