Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli

Venkat Gopalan, Ralph Golbik, Gideon Schreiber, Alan R. Fersht, Sidney Altman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Escherichia coli ribonuclease P (RNase P), a ribonucleoprotein complex which primarily functions in tRNA biosynthesis, is composed of a catalytic RNA subunit, M1 RNA, and a protein cofactor, C5 protein. The fluorescence emission spectrum of the single tryptophan residue-containing C5 protein exhibits maxima at 318 nm and 332 nm. Based on a comparison of the emission spectra of wild-type C5 protein and some of its mutant derivatives, we have determined that the 318 nm maximum could be the result of a complex formed in the excited state as a result of hydrophobic interactions between Trp109, Phe18 and Phe73. The analogous tryptophan fluorescence emission spectra of wild-type C5 protein and the barstar mutant W38F/W44F, taken together with the detailed structural information available for barstar, provide a possible explanation for the unusual emission spectrum of C5 protein.

Original languageEnglish (US)
Pages (from-to)765-769
Number of pages5
JournalJournal of Molecular Biology
Volume267
Issue number4
DOIs
StatePublished - Apr 11 1997
Externally publishedYes

Fingerprint

Ribonuclease P
Protein Subunits
Tryptophan
Fluorescence
Escherichia coli
Proteins
Catalytic RNA
Ribonucleoproteins
Mutant Proteins
Transfer RNA
Hydrophobic and Hydrophilic Interactions
Catalytic Domain
RNA

Keywords

  • Hydrophobic interactions
  • RNase P protein subunit
  • Trp fluorescence

ASJC Scopus subject areas

  • Virology

Cite this

Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli. / Gopalan, Venkat; Golbik, Ralph; Schreiber, Gideon; Fersht, Alan R.; Altman, Sidney.

In: Journal of Molecular Biology, Vol. 267, No. 4, 11.04.1997, p. 765-769.

Research output: Contribution to journalArticle

Gopalan, Venkat ; Golbik, Ralph ; Schreiber, Gideon ; Fersht, Alan R. ; Altman, Sidney. / Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli. In: Journal of Molecular Biology. 1997 ; Vol. 267, No. 4. pp. 765-769.
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