Flow Cytometry: Present and future

K. A. Muirheadi, P. K. Horan, G. Poste

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

Flow cytometry combines many of the advantages of microscopy and biochemical analysis in a single high precision technique for rapid phenotypic analysis and sorting of individual cells, microorganisms and cell organelles. Flow cytometry has evolved over the past twenty five years from a few custom-made instruments designed to meet individual research needs to commercially available instruments, used routinely in many areas of contemporary biomedical research, and for the diagnosis and staging of clinical disease. This review summarizes the principles, advantages and limitations and the major applications of flow cytometry in research and in clinical diagnosis, and outlines some of the opportunities and challenges for the further development of this versatile technology.

Original languageEnglish (US)
Pages (from-to)337-356
Number of pages20
JournalBio/Technology
Volume3
Issue number4
DOIs
StatePublished - Apr 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology

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