Flow-based microimmunoassay

Mark Hayes; N. A. Polson; A. N. Phayre; Antonio Garcia

doi:10.1021/ac0104680

Flow-based microimmunoassay

Mark Hayes, N. A. Polson, A. N. Phayre, Antonio Garcia

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

A small-volume heterogeneous immunoassay system is demonstrated in microchannels exploiting magnetic manipulations of small paramagnetic particles (1 - 2-μm diameter). The small-diameter particles help to create a high surface-area-to-volume ratio that generates the sensitivity for the small detection volumes. Flow characteristics of the magnetically formed packed bed within the channel allow the assay to be carried out quickly (minutes) while passing appropriate volumes of both samples and reagents (microliters to hundreds of nanoliters) through the system. The assay is demonstrated with a direct interaction of fluorescein isothiocyanate (FITC) with an immobilized anti-FITC conjugate in which a small-volume sample (< 1 μL) reaches 90% of maximum signal in 3 min. Heterogeneous sandwich assays are demonstrated with parathyroid hormone (PTH) and interleukin-5 (IL-5). Both the PTH assay and IL-5 assays were carried out on microliter volumes and demonstrated physiologically relevant sensitivity (∼μg/L).

Original language	English (US)
Pages (from-to)	5896-5902
Number of pages	7
Journal	Analytical Chemistry
Volume	73
Issue number	24
DOIs	https://doi.org/10.1021/ac0104680
State	Published - Dec 15 2001

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac0104680

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title = "Flow-based microimmunoassay",

abstract = "A small-volume heterogeneous immunoassay system is demonstrated in microchannels exploiting magnetic manipulations of small paramagnetic particles (1 - 2-μm diameter). The small-diameter particles help to create a high surface-area-to-volume ratio that generates the sensitivity for the small detection volumes. Flow characteristics of the magnetically formed packed bed within the channel allow the assay to be carried out quickly (minutes) while passing appropriate volumes of both samples and reagents (microliters to hundreds of nanoliters) through the system. The assay is demonstrated with a direct interaction of fluorescein isothiocyanate (FITC) with an immobilized anti-FITC conjugate in which a small-volume sample (< 1 μL) reaches 90% of maximum signal in 3 min. Heterogeneous sandwich assays are demonstrated with parathyroid hormone (PTH) and interleukin-5 (IL-5). Both the PTH assay and IL-5 assays were carried out on microliter volumes and demonstrated physiologically relevant sensitivity (∼μg/L).",

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T1 - Flow-based microimmunoassay

AU - Hayes, Mark

AU - Polson, N. A.

AU - Phayre, A. N.

AU - Garcia, Antonio

PY - 2001/12/15

Y1 - 2001/12/15

N2 - A small-volume heterogeneous immunoassay system is demonstrated in microchannels exploiting magnetic manipulations of small paramagnetic particles (1 - 2-μm diameter). The small-diameter particles help to create a high surface-area-to-volume ratio that generates the sensitivity for the small detection volumes. Flow characteristics of the magnetically formed packed bed within the channel allow the assay to be carried out quickly (minutes) while passing appropriate volumes of both samples and reagents (microliters to hundreds of nanoliters) through the system. The assay is demonstrated with a direct interaction of fluorescein isothiocyanate (FITC) with an immobilized anti-FITC conjugate in which a small-volume sample (< 1 μL) reaches 90% of maximum signal in 3 min. Heterogeneous sandwich assays are demonstrated with parathyroid hormone (PTH) and interleukin-5 (IL-5). Both the PTH assay and IL-5 assays were carried out on microliter volumes and demonstrated physiologically relevant sensitivity (∼μg/L).

AB - A small-volume heterogeneous immunoassay system is demonstrated in microchannels exploiting magnetic manipulations of small paramagnetic particles (1 - 2-μm diameter). The small-diameter particles help to create a high surface-area-to-volume ratio that generates the sensitivity for the small detection volumes. Flow characteristics of the magnetically formed packed bed within the channel allow the assay to be carried out quickly (minutes) while passing appropriate volumes of both samples and reagents (microliters to hundreds of nanoliters) through the system. The assay is demonstrated with a direct interaction of fluorescein isothiocyanate (FITC) with an immobilized anti-FITC conjugate in which a small-volume sample (< 1 μL) reaches 90% of maximum signal in 3 min. Heterogeneous sandwich assays are demonstrated with parathyroid hormone (PTH) and interleukin-5 (IL-5). Both the PTH assay and IL-5 assays were carried out on microliter volumes and demonstrated physiologically relevant sensitivity (∼μg/L).

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JF - Analytical Chemistry

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ER -

Flow-based microimmunoassay

Abstract

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