@article{563c437420794a10837883319ccec764,
title = "Fixed-target protein serial microcrystallography with an X-ray free electron laser",
abstract = "We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (∼2.5 {\AA}) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using X-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ∼38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before- destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods.",
author = "Hunter, {Mark S.} and Brent Segelke and Marc Messerschmidt and Williams, {Garth J.} and Nadia Zatsepin and Anton Barty and {Henry Benner}, W. and Carlson, {David B.} and Matthew Coleman and Alexander Graf and Hau-Riege, {Stefan P.} and Tommaso Pardini and {Marvin Seibert}, M. and James Evans and S{\'e}bastien Boutet and Matthias Frank",
note = "Funding Information: Work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344 and Pacific Northwest National Laboratory (operated by Battelle Memorial Institute) under Contract DE-AC05-76RL01830 and carried out at the Linac Coherent Light Source (LCLS) at SLAC National Accelerator Laboratory. LCLS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University. Support was provided by the UCOP Lab Fee Program (award no 118036), the LLNL Laboratory Directed Research and Development (LDRD) program under project 12-ERD-031, and the National Science Foundation under project MCB 1021577. We would like to thank G. M. Stewart and SLAC Infomedia for generating Fig. 1.",
year = "2014",
month = aug,
day = "12",
doi = "10.1038/srep06026",
language = "English (US)",
volume = "4",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}