Abstract

Previous measurements of the electronic conductance of DNA nucleotides or amino acids have used tunnel junctions in which the gap is mechanically adjusted, such as scanning tunneling microscopes or mechanically controllable break junctions. Fixed-junction devices have, at best, detected the passage of whole DNA molecules without yielding chemical information. Here, we report on a layered tunnel junction in which the tunnel gap is defined by a dielectric layer, deposited by atomic layer deposition. Reactive ion etching is used to drill a hole through the layers so that the tunnel junction can be exposed to molecules in solution. When the metal electrodes are functionalized with recognition molecules that capture DNA nucleotides via hydrogen bonds, the identities of the individual nucleotides are revealed by characteristic features of the fluctuating tunnel current associated with single-molecule binding events.

Original languageEnglish (US)
Pages (from-to)11994-12003
Number of pages10
JournalACS Nano
Volume8
Issue number12
DOIs
StatePublished - Dec 23 2014

Fingerprint

Tunnel junctions
nucleotides
Nucleotides
tunnel junctions
DNA
deoxyribonucleic acid
Molecules
tunnels
molecules
Tunnels
Atomic layer deposition
Reactive ion etching
atomic layer epitaxy
amino acids
Amino acids
Hydrogen bonds
Microscopes
Metals
microscopes
etching

Keywords

  • chemical recognition
  • DNA sequencing
  • recognition tunneling
  • tunnel junction

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Pang, P., Ashcroft, B. A., Song, W., Zhang, P., Biswas, S., Qing, Q., ... Lindsay, S. (2014). Fixed-gap tunnel junction for reading DNA nucleotides. ACS Nano, 8(12), 11994-12003. https://doi.org/10.1021/nn505356g

Fixed-gap tunnel junction for reading DNA nucleotides. / Pang, Pei; Ashcroft, Brian Alan; Song, Weisi; Zhang, Peiming; Biswas, Sovan; Qing, Quan; Yang, Jialing; Nemanich, Robert; Bai, Jingwei; Smith, Joshua T.; Reuter, Kathleen; Balagurusamy, Venkat S K; Astier, Yann; Stolovitzky, Gustavo; Lindsay, Stuart.

In: ACS Nano, Vol. 8, No. 12, 23.12.2014, p. 11994-12003.

Research output: Contribution to journalArticle

Pang, P, Ashcroft, BA, Song, W, Zhang, P, Biswas, S, Qing, Q, Yang, J, Nemanich, R, Bai, J, Smith, JT, Reuter, K, Balagurusamy, VSK, Astier, Y, Stolovitzky, G & Lindsay, S 2014, 'Fixed-gap tunnel junction for reading DNA nucleotides', ACS Nano, vol. 8, no. 12, pp. 11994-12003. https://doi.org/10.1021/nn505356g
Pang P, Ashcroft BA, Song W, Zhang P, Biswas S, Qing Q et al. Fixed-gap tunnel junction for reading DNA nucleotides. ACS Nano. 2014 Dec 23;8(12):11994-12003. https://doi.org/10.1021/nn505356g
Pang, Pei ; Ashcroft, Brian Alan ; Song, Weisi ; Zhang, Peiming ; Biswas, Sovan ; Qing, Quan ; Yang, Jialing ; Nemanich, Robert ; Bai, Jingwei ; Smith, Joshua T. ; Reuter, Kathleen ; Balagurusamy, Venkat S K ; Astier, Yann ; Stolovitzky, Gustavo ; Lindsay, Stuart. / Fixed-gap tunnel junction for reading DNA nucleotides. In: ACS Nano. 2014 ; Vol. 8, No. 12. pp. 11994-12003.
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