Fixed-gap tunnel junction for reading DNA nucleotides

Pei Pang, Brian Alan Ashcroft, Weisi Song, Peiming Zhang, Sovan Biswas, Quan Qing, Jialing Yang, Robert Nemanich, Jingwei Bai, Joshua T. Smith, Kathleen Reuter, Venkat S K Balagurusamy, Yann Astier, Gustavo Stolovitzky, Stuart Lindsay

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Previous measurements of the electronic conductance of DNA nucleotides or amino acids have used tunnel junctions in which the gap is mechanically adjusted, such as scanning tunneling microscopes or mechanically controllable break junctions. Fixed-junction devices have, at best, detected the passage of whole DNA molecules without yielding chemical information. Here, we report on a layered tunnel junction in which the tunnel gap is defined by a dielectric layer, deposited by atomic layer deposition. Reactive ion etching is used to drill a hole through the layers so that the tunnel junction can be exposed to molecules in solution. When the metal electrodes are functionalized with recognition molecules that capture DNA nucleotides via hydrogen bonds, the identities of the individual nucleotides are revealed by characteristic features of the fluctuating tunnel current associated with single-molecule binding events.

Original languageEnglish (US)
Pages (from-to)11994-12003
Number of pages10
JournalACS nano
Volume8
Issue number12
DOIs
StatePublished - Dec 23 2014

Keywords

  • DNA sequencing
  • chemical recognition
  • recognition tunneling
  • tunnel junction

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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    Pang, P., Ashcroft, B. A., Song, W., Zhang, P., Biswas, S., Qing, Q., Yang, J., Nemanich, R., Bai, J., Smith, J. T., Reuter, K., Balagurusamy, V. S. K., Astier, Y., Stolovitzky, G., & Lindsay, S. (2014). Fixed-gap tunnel junction for reading DNA nucleotides. ACS nano, 8(12), 11994-12003. https://doi.org/10.1021/nn505356g