Fine-scale mapping of the Nasonia genome to chromosomes using a high-density genotyping microarray

Christopher A. Desjardins, Juergen Gadau, Jacqueline A. Lopez, Oliver Niehuis, Amanda R. Avery, David W. Loehlin, Stephen Richards, John K. Colbourne, John H. Werren

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nasonia, a genus of four closely related parasitoid insect species, is a model system for genetic research. Their haplodiploid genetics (haploid males and diploid females) and interfertile species are advantageous for the genetic analysis of complex traits and the genetic basis of species differences. A finescale genomic map is an important tool for advancing genetic studies in this system. We developed and used a hybrid genotyping microarray to generate a high-resolution genetic map that covers 79% of the sequenced genome of Nasonia vitripennis. The microarray is based on differential hybridization of speciesspecific oligos between N. vitripennis and Nasonia giraulti at more than 20,000 markers spanning the Nasonia genome. The map places 729 scaffolds onto the five linkage groups of Nasonia, including locating many smaller scaffolds that would be difficult to map by other means. The microarray was used to characterize 26 segmental introgression lines containing chromosomal regions from one species in the genetic background of another. These segmental introgression lines have been used for rapid screening and mapping of quantitative trait loci involved in species differences. Finally, the microarray is extended to bulk-segregant analysis and genotyping of other Nasonia species combinations. These resources should further expand the usefulness of Nasonia for studies of the genetic basis and architecture of complex traits and speciation.

Original languageEnglish (US)
Pages (from-to)205-215
Number of pages11
JournalG3: Genes, Genomes, Genetics
Volume3
Issue number2
DOIs
StatePublished - 2013

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Chromosome Mapping
Chromosomes
Genome
Genetic Research
Quantitative Trait Loci
Haploidy
Diploidy
Insects
Genetic Background

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Desjardins, C. A., Gadau, J., Lopez, J. A., Niehuis, O., Avery, A. R., Loehlin, D. W., ... Werren, J. H. (2013). Fine-scale mapping of the Nasonia genome to chromosomes using a high-density genotyping microarray. G3: Genes, Genomes, Genetics, 3(2), 205-215. https://doi.org/10.1534/g3.112.004739

Fine-scale mapping of the Nasonia genome to chromosomes using a high-density genotyping microarray. / Desjardins, Christopher A.; Gadau, Juergen; Lopez, Jacqueline A.; Niehuis, Oliver; Avery, Amanda R.; Loehlin, David W.; Richards, Stephen; Colbourne, John K.; Werren, John H.

In: G3: Genes, Genomes, Genetics, Vol. 3, No. 2, 2013, p. 205-215.

Research output: Contribution to journalArticle

Desjardins, CA, Gadau, J, Lopez, JA, Niehuis, O, Avery, AR, Loehlin, DW, Richards, S, Colbourne, JK & Werren, JH 2013, 'Fine-scale mapping of the Nasonia genome to chromosomes using a high-density genotyping microarray', G3: Genes, Genomes, Genetics, vol. 3, no. 2, pp. 205-215. https://doi.org/10.1534/g3.112.004739
Desjardins, Christopher A. ; Gadau, Juergen ; Lopez, Jacqueline A. ; Niehuis, Oliver ; Avery, Amanda R. ; Loehlin, David W. ; Richards, Stephen ; Colbourne, John K. ; Werren, John H. / Fine-scale mapping of the Nasonia genome to chromosomes using a high-density genotyping microarray. In: G3: Genes, Genomes, Genetics. 2013 ; Vol. 3, No. 2. pp. 205-215.
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