Field ionization mass spectrometry—II: Benzyloxycarbonyl and t‐butyloxycarbonyl derivatives of some simple peptides

Peter Brown, George Pettit

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A brief discussion is given of some factors that merit consideration in the analysis of peptides by mass spectrometry, with emphasis on sequence determination. The first systematic study of simple peptides by field ionization (FI) is described, and comparison made with the corresponding electron‐impact (EI) ionization spectra, both at low resolution (approx. 1500). The substances examined were either benzyloxycarbonyl or t‐butyloxycarbonyl derivatives of di‐through pentapeptide methyl esters, containing the amino acids glycine, alanine, leucine, serine, threonine, proline and tyrosine. The incidences of sequence‐characteristic cleavage peaks and rearrangement ion peaks were both slightly lower in the FI than the EI spectra, although the peak relative intensities generally were higher under FI conditions.

Original languageEnglish (US)
Pages (from-to)67-83
Number of pages17
JournalOrganic Mass Spectrometry
Volume3
Issue number1
DOIs
StatePublished - 1970

Fingerprint

Peptides
Ionization
peptides
Derivatives
ionization
Threonine
Proline
Leucine
Alanine
Glycine
Serine
Sequence Analysis
Tyrosine
Amino acids
Mass Spectrometry
Esters
Ions
Amino Acids
leucine
tyrosine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Instrumentation

Cite this

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