Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives

Anne Dell, Howard R. Morris, Mark D. Levin, Sidney M. Hecht

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Earlier work using field desorption mass spectrometry for the characterisation of guanidino containing bleomycins is extended to include bleomycin A2 and related substances. The new technique of fast atom bombardment mass spectrometry is assessed and compared with field desorption for the analysis of these complex antibiotics. Bleomycin A2 is shown to be more reactive to acetic anhydride than bleomycin B2. A procedure for in situ derivatisation for fast atom bombardment studies is described and unexpected catalysis in the glycerol matrix is reported.

Original languageEnglish (US)
Pages (from-to)730-738
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume102
Issue number2
DOIs
StatePublished - Sep 30 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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