Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives

Anne Dell; Howard R. Morris; Mark D. Levin; Sidney M. Hecht

doi:10.1016/S0006-291X(81)80193-3

Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives

Anne Dell, Howard R. Morris, Mark D. Levin, Sidney M. Hecht

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Earlier work using field desorption mass spectrometry for the characterisation of guanidino containing bleomycins is extended to include bleomycin A₂ and related substances. The new technique of fast atom bombardment mass spectrometry is assessed and compared with field desorption for the analysis of these complex antibiotics. Bleomycin A₂ is shown to be more reactive to acetic anhydride than bleomycin B₂. A procedure for in situ derivatisation for fast atom bombardment studies is described and unexpected catalysis in the glycerol matrix is reported.

Original language	English (US)
Pages (from-to)	730-738
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	102
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(81)80193-3
State	Published - Sep 30 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives",

abstract = "Earlier work using field desorption mass spectrometry for the characterisation of guanidino containing bleomycins is extended to include bleomycin A2 and related substances. The new technique of fast atom bombardment mass spectrometry is assessed and compared with field desorption for the analysis of these complex antibiotics. Bleomycin A2 is shown to be more reactive to acetic anhydride than bleomycin B2. A procedure for in situ derivatisation for fast atom bombardment studies is described and unexpected catalysis in the glycerol matrix is reported.",

author = "Anne Dell and Morris, {Howard R.} and Levin, {Mark D.} and Hecht, {Sidney M.}",

note = "Funding Information: This work was supported at Imperial College by the Medical and Science Research Councils and at the University of Virginia by PHS Research Grant Number CA27603, awarded by the National Cancer Institute;",

year = "1981",

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doi = "10.1016/S0006-291X(81)80193-3",

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T1 - Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives

AU - Dell, Anne

AU - Morris, Howard R.

AU - Levin, Mark D.

AU - Hecht, Sidney M.

N1 - Funding Information: This work was supported at Imperial College by the Medical and Science Research Councils and at the University of Virginia by PHS Research Grant Number CA27603, awarded by the National Cancer Institute;

PY - 1981/9/30

Y1 - 1981/9/30

N2 - Earlier work using field desorption mass spectrometry for the characterisation of guanidino containing bleomycins is extended to include bleomycin A2 and related substances. The new technique of fast atom bombardment mass spectrometry is assessed and compared with field desorption for the analysis of these complex antibiotics. Bleomycin A2 is shown to be more reactive to acetic anhydride than bleomycin B2. A procedure for in situ derivatisation for fast atom bombardment studies is described and unexpected catalysis in the glycerol matrix is reported.

AB - Earlier work using field desorption mass spectrometry for the characterisation of guanidino containing bleomycins is extended to include bleomycin A2 and related substances. The new technique of fast atom bombardment mass spectrometry is assessed and compared with field desorption for the analysis of these complex antibiotics. Bleomycin A2 is shown to be more reactive to acetic anhydride than bleomycin B2. A procedure for in situ derivatisation for fast atom bombardment studies is described and unexpected catalysis in the glycerol matrix is reported.

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JO - Biochemical and Biophysical Research Communications

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Field desorption and fast atom bombardment mass spectrometry of bleomycins and their derivatives

Abstract

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