Fibroblast functionality on novel Ti30Ta nanotube array

Patricia Capellato, Barbara Smith, Ketul C. Popat, Ana P R Alves Claro

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H 2SO 4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.

Original languageEnglish (US)
Pages (from-to)2060-2067
Number of pages8
JournalMaterials Science and Engineering C
Volume32
Issue number7
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Fingerprint

fibroblasts
Fibroblasts
Nanotubes
nanotubes
Imaging techniques
Scanning electron microscopy
scanning electron microscopy
Dimethyl sulfoxide
biocompatibility
Angle measurement
Dimethyl Sulfoxide
Biocompatibility
viability
surface properties
Electrolytes
Contact angle
Surface properties
Energy dispersive spectroscopy
Assays
Materials properties

Keywords

  • Anodization
  • Human dermal fibroblasts
  • Ti30Ta nanotubes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Fibroblast functionality on novel Ti30Ta nanotube array. / Capellato, Patricia; Smith, Barbara; Popat, Ketul C.; Claro, Ana P R Alves.

In: Materials Science and Engineering C, Vol. 32, No. 7, 01.10.2012, p. 2060-2067.

Research output: Contribution to journalArticle

Capellato, Patricia ; Smith, Barbara ; Popat, Ketul C. ; Claro, Ana P R Alves. / Fibroblast functionality on novel Ti30Ta nanotube array. In: Materials Science and Engineering C. 2012 ; Vol. 32, No. 7. pp. 2060-2067.
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