FGF-2-induced cell proliferation stimulates anatomical neurophysiological and functional recovery from neonatal motor cortex injury

Marie H. Monfils, Ira Driscoll, Holly Kamitakahara, Brett Wilson, Corey Flynn, G. Campbell Teskey, Jeffrey Kleim, Bryan Kolb

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Infant rats treated with basic fibroblast growth factor-2 (FGF-2) after postnatal day (P)10 motor cortical injury, show functional improvement in adulthood relative to those that do not receive FGF-2. In this study we used a combination of behavioural, immunohistochemical, electrophysiological, electron microscopic and teratological approaches to investigate possible mechanisms by which FGF-2 may influence functional recovery. We show that subcutaneous injections of FGF-2 following bilateral lesions to the motor cortex at P10 in the rat leads to filling of the lesion area with migrating neuroblasts and cycling cells. We assessed the functionality of this tissue in adulthood, and show that cells from the filled region spontaneously fire and form synapses. Behavioural analysis shows enhanced motor performance in the FGF-2-treated lesion rats in comparison to vehicle-treated lesion rats, and this improvement is reversed by removal of the tissue from the previously lesioned area or by blocking cortical regeneration by embryonic treatment with bromodeoxyuridine (BrdU). The results show that FGF-2 stimulates filling of the lesion cavity with cells after neonatal motor cortex lesions, that the new tissue has anatomical and physiological properties similar to control tissue, and that the filled region supports motor behaviour.

Original languageEnglish (US)
Pages (from-to)739-749
Number of pages11
JournalEuropean Journal of Neuroscience
Volume24
Issue number3
DOIs
StatePublished - Aug 2006
Externally publishedYes

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Motor Cortex
Fibroblast Growth Factor 2
Cell Proliferation
Wounds and Injuries
Bromodeoxyuridine
Subcutaneous Injections
Synapses
Regeneration
Electrons

Keywords

  • Doublecortin
  • Electron microscopy
  • Ki67
  • Multi-unit
  • Reaching

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

FGF-2-induced cell proliferation stimulates anatomical neurophysiological and functional recovery from neonatal motor cortex injury. / Monfils, Marie H.; Driscoll, Ira; Kamitakahara, Holly; Wilson, Brett; Flynn, Corey; Teskey, G. Campbell; Kleim, Jeffrey; Kolb, Bryan.

In: European Journal of Neuroscience, Vol. 24, No. 3, 08.2006, p. 739-749.

Research output: Contribution to journalArticle

Monfils, Marie H. ; Driscoll, Ira ; Kamitakahara, Holly ; Wilson, Brett ; Flynn, Corey ; Teskey, G. Campbell ; Kleim, Jeffrey ; Kolb, Bryan. / FGF-2-induced cell proliferation stimulates anatomical neurophysiological and functional recovery from neonatal motor cortex injury. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 3. pp. 739-749.
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