Fetal wound healing: An in vitro explant model

D. Andrew R Burd, Michael T. Longaker, N. Scott Adzick, Carolyn Compton, Michael R. Harrison, John W. Siebert, H. Paul Ehrlich

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ability of fetal skin wounds to heal without scar formation is remarkable. The mechanisms that endow the fetus with this unique healing ability remain unknown. We have developed an in vitro explant model using fetal sheep skin to investigate fetal wound healing. This model eliminates the complex systemic mechanisms that modulate in vivo wound healing. We demonstrated that using an enriched medium, midgestation fetal sheep skin explants following wounding reepithelialized within 4 days. By 7 days after wounding the confluent epidermis was thicker, but the dermal wound remained open. This model demonstrates that it is possible to achieve conditions in culture that maintain tissue viability and support reepithelialization. This model may allow us to resolve some of the individual components that participate in the process of scarless fetal skin healing.

Original languageEnglish (US)
Pages (from-to)898-901
Number of pages4
JournalJournal of Pediatric Surgery
Volume25
Issue number8
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Wound Healing
Skin
Sheep
Tissue Survival
Wounds and Injuries
Epidermis
Cicatrix
Fetus
In Vitro Techniques

Keywords

  • Fetal wound healing

ASJC Scopus subject areas

  • Surgery

Cite this

Burd, D. A. R., Longaker, M. T., Adzick, N. S., Compton, C., Harrison, M. R., Siebert, J. W., & Ehrlich, H. P. (1990). Fetal wound healing: An in vitro explant model. Journal of Pediatric Surgery, 25(8), 898-901. https://doi.org/10.1016/0022-3468(90)90199-J

Fetal wound healing : An in vitro explant model. / Burd, D. Andrew R; Longaker, Michael T.; Adzick, N. Scott; Compton, Carolyn; Harrison, Michael R.; Siebert, John W.; Ehrlich, H. Paul.

In: Journal of Pediatric Surgery, Vol. 25, No. 8, 1990, p. 898-901.

Research output: Contribution to journalArticle

Burd, DAR, Longaker, MT, Adzick, NS, Compton, C, Harrison, MR, Siebert, JW & Ehrlich, HP 1990, 'Fetal wound healing: An in vitro explant model', Journal of Pediatric Surgery, vol. 25, no. 8, pp. 898-901. https://doi.org/10.1016/0022-3468(90)90199-J
Burd DAR, Longaker MT, Adzick NS, Compton C, Harrison MR, Siebert JW et al. Fetal wound healing: An in vitro explant model. Journal of Pediatric Surgery. 1990;25(8):898-901. https://doi.org/10.1016/0022-3468(90)90199-J
Burd, D. Andrew R ; Longaker, Michael T. ; Adzick, N. Scott ; Compton, Carolyn ; Harrison, Michael R. ; Siebert, John W. ; Ehrlich, H. Paul. / Fetal wound healing : An in vitro explant model. In: Journal of Pediatric Surgery. 1990 ; Vol. 25, No. 8. pp. 898-901.
@article{99f7fd4d02404ddd8ba659d2893e3e0f,
title = "Fetal wound healing: An in vitro explant model",
abstract = "The ability of fetal skin wounds to heal without scar formation is remarkable. The mechanisms that endow the fetus with this unique healing ability remain unknown. We have developed an in vitro explant model using fetal sheep skin to investigate fetal wound healing. This model eliminates the complex systemic mechanisms that modulate in vivo wound healing. We demonstrated that using an enriched medium, midgestation fetal sheep skin explants following wounding reepithelialized within 4 days. By 7 days after wounding the confluent epidermis was thicker, but the dermal wound remained open. This model demonstrates that it is possible to achieve conditions in culture that maintain tissue viability and support reepithelialization. This model may allow us to resolve some of the individual components that participate in the process of scarless fetal skin healing.",
keywords = "Fetal wound healing",
author = "Burd, {D. Andrew R} and Longaker, {Michael T.} and Adzick, {N. Scott} and Carolyn Compton and Harrison, {Michael R.} and Siebert, {John W.} and Ehrlich, {H. Paul}",
year = "1990",
doi = "10.1016/0022-3468(90)90199-J",
language = "English (US)",
volume = "25",
pages = "898--901",
journal = "Journal of Pediatric Surgery",
issn = "0022-3468",
publisher = "W.B. Saunders Ltd",
number = "8",

}

TY - JOUR

T1 - Fetal wound healing

T2 - An in vitro explant model

AU - Burd, D. Andrew R

AU - Longaker, Michael T.

AU - Adzick, N. Scott

AU - Compton, Carolyn

AU - Harrison, Michael R.

AU - Siebert, John W.

AU - Ehrlich, H. Paul

PY - 1990

Y1 - 1990

N2 - The ability of fetal skin wounds to heal without scar formation is remarkable. The mechanisms that endow the fetus with this unique healing ability remain unknown. We have developed an in vitro explant model using fetal sheep skin to investigate fetal wound healing. This model eliminates the complex systemic mechanisms that modulate in vivo wound healing. We demonstrated that using an enriched medium, midgestation fetal sheep skin explants following wounding reepithelialized within 4 days. By 7 days after wounding the confluent epidermis was thicker, but the dermal wound remained open. This model demonstrates that it is possible to achieve conditions in culture that maintain tissue viability and support reepithelialization. This model may allow us to resolve some of the individual components that participate in the process of scarless fetal skin healing.

AB - The ability of fetal skin wounds to heal without scar formation is remarkable. The mechanisms that endow the fetus with this unique healing ability remain unknown. We have developed an in vitro explant model using fetal sheep skin to investigate fetal wound healing. This model eliminates the complex systemic mechanisms that modulate in vivo wound healing. We demonstrated that using an enriched medium, midgestation fetal sheep skin explants following wounding reepithelialized within 4 days. By 7 days after wounding the confluent epidermis was thicker, but the dermal wound remained open. This model demonstrates that it is possible to achieve conditions in culture that maintain tissue viability and support reepithelialization. This model may allow us to resolve some of the individual components that participate in the process of scarless fetal skin healing.

KW - Fetal wound healing

UR - http://www.scopus.com/inward/record.url?scp=0025029774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025029774&partnerID=8YFLogxK

U2 - 10.1016/0022-3468(90)90199-J

DO - 10.1016/0022-3468(90)90199-J

M3 - Article

C2 - 2401945

AN - SCOPUS:0025029774

VL - 25

SP - 898

EP - 901

JO - Journal of Pediatric Surgery

JF - Journal of Pediatric Surgery

SN - 0022-3468

IS - 8

ER -