FertilizationInduced changes in the vitelline envelope of echinoderm and amphibian eggs: Selfassembly of an extracellular matrix

C. Larabell, D. E. Chandler

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The surface of the unfertilized sea urchin egg is covered by the vitelline layer (VL), a fibrous extracellular matrix that contains receptors for sperm. At fertilization, cortical granule exocytosis releases enzymes and structural proteins that cause the VL to elevate and become remodelled into the mechanically and chemically tough fertilization envelope. This envelope prevents further penetration of sperm and protects the embryo during early development. A thicker, more complex vitelline envelope surrounds the Xenopus laevis egg. This fibrous coat is also restructured at fertilization to produce an impenetrable barrier to sperm. The biochemical steps that occur during selfassembly of these fertilization envelopes are reviewed, and the ultrastructural changes that occur, as seen in platinum replicas of quickfrozen, deepetched, and rotaryshadowed eggs, are illustrated. Copyright1991 WileyLiss, Inc.

Original languageEnglish (US)
Pages (from-to)294-318
Number of pages25
JournalJournal of electron microscopy technique
Volume17
Issue number3
DOIs
StatePublished - Mar 1991

Keywords

  • Quick freezing
  • Sea urchin egg
  • Xenopus laevis egg

ASJC Scopus subject areas

  • Anatomy

Fingerprint Dive into the research topics of 'FertilizationInduced changes in the vitelline envelope of echinoderm and amphibian eggs: Selfassembly of an extracellular matrix'. Together they form a unique fingerprint.

  • Cite this