Ferritin RNA interference inhibits the formation of iron granules in the trophocytes of worker honey bees (Apis mellifera)

Chin Yuan Hsu, Hsiao Fan Lo, Navdeep S. Mutti, Gro Amdam

Research output: Contribution to journalArticle

Abstract

Iron granules containing superparamagnetic magnetite act as magnetoreceptor for magnetoreception in honey bees. Biomineralization of iron granules occurs in the iron deposition vesicles of trophocytes and requires the participation of actin, myosin, ferritin2, and ATP synthase. The mechanism of magnetoreception in honey bees can be explored by suppressing the formation of iron granules. Toward this goal, we injected double-stranded RNA of ferritin2 and ferritin1 into newly emerged worker honey bees to knock down these genes via RNA interference. We confirmed that mRNA and protein production of the ferritins was inhibited, leading to immature iron granules. Downregulating ferritin2 and ferritin1, moreover, leads to different deposition morphology of 7.5-nm diameter iron particles, indicating that the two genes play different roles in the formation of iron granules in worker honey bees.

Original languageEnglish (US)
Article number10098
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Honey
Bees
Ferritins
RNA Interference
Iron
Ferrosoferric Oxide
Gene Knockdown Techniques
Double-Stranded RNA
Myosins
Actins
Down-Regulation
Adenosine Triphosphate
Messenger RNA
Genes

ASJC Scopus subject areas

  • General

Cite this

Ferritin RNA interference inhibits the formation of iron granules in the trophocytes of worker honey bees (Apis mellifera). / Hsu, Chin Yuan; Lo, Hsiao Fan; Mutti, Navdeep S.; Amdam, Gro.

In: Scientific reports, Vol. 9, No. 1, 10098, 01.12.2019.

Research output: Contribution to journalArticle

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