Ferritin from the obligate anaerobe Porphyromonas gingivalis: Purification, gene cloning and mutant studies

Dinath B. Ratnayake, Sun Nyunt Wai, Yixin Shi, Kazunobu Amako, Hiroaki Nakayama, Koji Nakayama

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Porphyromonas gingivalis is an obligate anaerobe that utilizes haem, transferrin and haemoglobin efficiently as sources of iron for growth, and has the ability to store haem on its cell surface, resulting in black pigmentation of colonies on blood agar plates. However, little is known about intracellular iron storage in this organism. Ferritin is one of the intracellular iron-storage proteins and may also contribute to the protection of organisms against oxidative stresses generated by intracellular free iron. A ferritin-like protein was purified from P. gingivalis and the encoding gene (ftn) was cloned from chromosomal DNA using information on its amino-terminal amino acid sequence. Comparison of the amino acid sequence deduced from the nucleotide sequence of ftn with those of known ferritins and bacterioferritins identified the protein as a ferritin and positioned it between proteins from the Proteobacteria and Thermotogales. The P. gingivalis ferritin was found to contain non-haem iron, thus confirming its identity. Construction and characterization of a P. gingivalis ferritin-deficient mutant revealed that the ferritin was particularly important for the bacterium to survive under iron-depleted conditions (both haemin and transferrin starvation), indicating that intracellular iron is stored in ferritin regardless of the iron source and that the iron stored in ferritin is utilized under iron-restricted conditions. However, the ferritin appeared not to contribute to protection against oxidative stresses caused by peroxides and atmospheric oxygen.

Original languageEnglish (US)
Pages (from-to)1119-1127
Number of pages9
JournalMicrobiology
Volume146
Issue number5
StatePublished - May 2000
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Ferritins
Organism Cloning
Iron
Genes
Transferrin
Heme
Amino Acid Sequence
Proteins
Oxidative Stress
Proteobacteria
Hemin
Peroxides
Pigmentation
Starvation
Agar
Hemoglobins
Oxygen
Bacteria

Keywords

  • Ferritin
  • Iron storage
  • Oxidative stress
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Microbiology

Cite this

Ratnayake, D. B., Wai, S. N., Shi, Y., Amako, K., Nakayama, H., & Nakayama, K. (2000). Ferritin from the obligate anaerobe Porphyromonas gingivalis: Purification, gene cloning and mutant studies. Microbiology, 146(5), 1119-1127.

Ferritin from the obligate anaerobe Porphyromonas gingivalis : Purification, gene cloning and mutant studies. / Ratnayake, Dinath B.; Wai, Sun Nyunt; Shi, Yixin; Amako, Kazunobu; Nakayama, Hiroaki; Nakayama, Koji.

In: Microbiology, Vol. 146, No. 5, 05.2000, p. 1119-1127.

Research output: Contribution to journalArticle

Ratnayake, DB, Wai, SN, Shi, Y, Amako, K, Nakayama, H & Nakayama, K 2000, 'Ferritin from the obligate anaerobe Porphyromonas gingivalis: Purification, gene cloning and mutant studies', Microbiology, vol. 146, no. 5, pp. 1119-1127.
Ratnayake DB, Wai SN, Shi Y, Amako K, Nakayama H, Nakayama K. Ferritin from the obligate anaerobe Porphyromonas gingivalis: Purification, gene cloning and mutant studies. Microbiology. 2000 May;146(5):1119-1127.
Ratnayake, Dinath B. ; Wai, Sun Nyunt ; Shi, Yixin ; Amako, Kazunobu ; Nakayama, Hiroaki ; Nakayama, Koji. / Ferritin from the obligate anaerobe Porphyromonas gingivalis : Purification, gene cloning and mutant studies. In: Microbiology. 2000 ; Vol. 146, No. 5. pp. 1119-1127.
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