Femtosecond X-ray diffraction from two-dimensional protein crystals

Matthias Frank, David B. Carlson, Mark S. Hunter, Garth J. Williams, Marc Messerschmidt, Nadia Zatsepin, Anton Barty, W. Henry Benner, Kaiqin Chu, Alexander T. Graf, Stefan P. Hau-Riege, Richard Kirian, Celestino Padeste, Tommaso Pardini, Bill Pedrini, Brent Segelke, M. Marvin Seibert, John Spence, Ching Ju Tsai, Stephen M. Lane & 5 others Xiao Dan Li, Gebhard Schertler, Sebastien Boutet, Matthew Coleman, James E. Evans

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    X-ray diffraction patterns from two-dimensional (2-D) protein crystals obtained using femtosecond X-ray pulses from an X-ray free-electron laser (XFEL) are presented. To date, it has not been possible to acquire transmission X-ray diffraction patterns from individual 2-D protein crystals due to radiation damage. However, the intense and ultrafast pulses generated by an XFEL permit a new method of collecting diffraction data before the sample is destroyed. Utilizing a diffract-before-destroy approach at the Linac Coherent Light Source, Bragg diffraction was acquired to better than 8.5Å resolution for two different 2-D protein crystal samples each less than 10nm thick and maintained at room temperature. These proof-of-principle results show promise for structural analysis of both soluble and membrane proteins arranged as 2-D crystals without requiring cryogenic conditions or the formation of three-dimensional crystals.

    Original languageEnglish (US)
    Pages (from-to)95-100
    Number of pages6
    JournalIUCrJ
    Volume1
    DOIs
    StatePublished - Feb 28 2014

    Fingerprint

    X-Ray Diffraction
    X-Rays
    proteins
    Proteins
    X ray diffraction
    Crystals
    X ray lasers
    Lasers
    diffraction
    Electrons
    crystals
    Free electron lasers
    x rays
    free electron lasers
    Diffraction patterns
    Laser pulses
    diffraction patterns
    Diffraction
    Membrane Proteins
    Radiation

    Keywords

    • femtosecond crystallography
    • membrane protein
    • single layer X-ray diffraction
    • two-dimensional protein crystal

    ASJC Scopus subject areas

    • Chemistry(all)
    • Materials Science(all)
    • Condensed Matter Physics
    • Biochemistry

    Cite this

    Frank, M., Carlson, D. B., Hunter, M. S., Williams, G. J., Messerschmidt, M., Zatsepin, N., ... Evans, J. E. (2014). Femtosecond X-ray diffraction from two-dimensional protein crystals. IUCrJ, 1, 95-100. https://doi.org/10.1107/S2052252514001444

    Femtosecond X-ray diffraction from two-dimensional protein crystals. / Frank, Matthias; Carlson, David B.; Hunter, Mark S.; Williams, Garth J.; Messerschmidt, Marc; Zatsepin, Nadia; Barty, Anton; Benner, W. Henry; Chu, Kaiqin; Graf, Alexander T.; Hau-Riege, Stefan P.; Kirian, Richard; Padeste, Celestino; Pardini, Tommaso; Pedrini, Bill; Segelke, Brent; Seibert, M. Marvin; Spence, John; Tsai, Ching Ju; Lane, Stephen M.; Li, Xiao Dan; Schertler, Gebhard; Boutet, Sebastien; Coleman, Matthew; Evans, James E.

    In: IUCrJ, Vol. 1, 28.02.2014, p. 95-100.

    Research output: Contribution to journalArticle

    Frank, M, Carlson, DB, Hunter, MS, Williams, GJ, Messerschmidt, M, Zatsepin, N, Barty, A, Benner, WH, Chu, K, Graf, AT, Hau-Riege, SP, Kirian, R, Padeste, C, Pardini, T, Pedrini, B, Segelke, B, Seibert, MM, Spence, J, Tsai, CJ, Lane, SM, Li, XD, Schertler, G, Boutet, S, Coleman, M & Evans, JE 2014, 'Femtosecond X-ray diffraction from two-dimensional protein crystals' IUCrJ, vol. 1, pp. 95-100. https://doi.org/10.1107/S2052252514001444
    Frank, Matthias ; Carlson, David B. ; Hunter, Mark S. ; Williams, Garth J. ; Messerschmidt, Marc ; Zatsepin, Nadia ; Barty, Anton ; Benner, W. Henry ; Chu, Kaiqin ; Graf, Alexander T. ; Hau-Riege, Stefan P. ; Kirian, Richard ; Padeste, Celestino ; Pardini, Tommaso ; Pedrini, Bill ; Segelke, Brent ; Seibert, M. Marvin ; Spence, John ; Tsai, Ching Ju ; Lane, Stephen M. ; Li, Xiao Dan ; Schertler, Gebhard ; Boutet, Sebastien ; Coleman, Matthew ; Evans, James E. / Femtosecond X-ray diffraction from two-dimensional protein crystals. In: IUCrJ. 2014 ; Vol. 1. pp. 95-100.
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