Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Salah Awel, Richard Kirian, Max O. Wiedorn, Kenneth R. Beyerlein, Nils Roth, Daniel A. Horke, Dominik Oberthür, Juraj Knoska, Valerio Mariani, Andrew Morgan, Luigi Adriano, Alexandra Tolstikova, P. Lourdu Xavier, Oleksandr Yefanov, Andrew Aquila, Anton Barty, Shatabdi Roy-Chowdhury, Mark S. Hunter, Daniel James, Joseph S. Robinson & 5 others Uwe Weierstall, Andrei V. Rode, Saša Bajt, Jochen Küpper, Henry N. Chapman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalJournal of Applied Crystallography
Volume51
Issue number1
DOIs
StatePublished - Feb 1 2018

Fingerprint

X-Ray Diffraction
Nanoparticles
Nanocrystals
Granulovirus
Jet Injections
Diffraction
X-Rays
X ray diffraction
Helium
Liquids
Vacuum
Aerosols
X ray lasers
Proteins
Lasers
Gases
Free electron lasers
Electrons
X ray scattering
Mountings

Keywords

  • aerosols
  • nanocrystals
  • X-ray diffraction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Awel, S., Kirian, R., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., ... Chapman, H. N. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Journal of Applied Crystallography, 51(1), 133-139. https://doi.org/10.1107/S1600576717018131

Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. / Awel, Salah; Kirian, Richard; Wiedorn, Max O.; Beyerlein, Kenneth R.; Roth, Nils; Horke, Daniel A.; Oberthür, Dominik; Knoska, Juraj; Mariani, Valerio; Morgan, Andrew; Adriano, Luigi; Tolstikova, Alexandra; Xavier, P. Lourdu; Yefanov, Oleksandr; Aquila, Andrew; Barty, Anton; Roy-Chowdhury, Shatabdi; Hunter, Mark S.; James, Daniel; Robinson, Joseph S.; Weierstall, Uwe; Rode, Andrei V.; Bajt, Saša; Küpper, Jochen; Chapman, Henry N.

In: Journal of Applied Crystallography, Vol. 51, No. 1, 01.02.2018, p. 133-139.

Research output: Contribution to journalArticle

Awel, S, Kirian, R, Wiedorn, MO, Beyerlein, KR, Roth, N, Horke, DA, Oberthür, D, Knoska, J, Mariani, V, Morgan, A, Adriano, L, Tolstikova, A, Xavier, PL, Yefanov, O, Aquila, A, Barty, A, Roy-Chowdhury, S, Hunter, MS, James, D, Robinson, JS, Weierstall, U, Rode, AV, Bajt, S, Küpper, J & Chapman, HN 2018, 'Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals', Journal of Applied Crystallography, vol. 51, no. 1, pp. 133-139. https://doi.org/10.1107/S1600576717018131
Awel, Salah ; Kirian, Richard ; Wiedorn, Max O. ; Beyerlein, Kenneth R. ; Roth, Nils ; Horke, Daniel A. ; Oberthür, Dominik ; Knoska, Juraj ; Mariani, Valerio ; Morgan, Andrew ; Adriano, Luigi ; Tolstikova, Alexandra ; Xavier, P. Lourdu ; Yefanov, Oleksandr ; Aquila, Andrew ; Barty, Anton ; Roy-Chowdhury, Shatabdi ; Hunter, Mark S. ; James, Daniel ; Robinson, Joseph S. ; Weierstall, Uwe ; Rode, Andrei V. ; Bajt, Saša ; Küpper, Jochen ; Chapman, Henry N. / Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. In: Journal of Applied Crystallography. 2018 ; Vol. 51, No. 1. pp. 133-139.
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AU - James, Daniel

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AU - Weierstall, Uwe

AU - Rode, Andrei V.

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AU - Küpper, Jochen

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