Abstract
X-ray diffraction patterns may be obtained from individual submicron protein nanocrystals using a femtosecond pulse from a free-electron X-ray laser. Many single-shot patterns are read out every second from a stream of nanocrystals lying in random orientations. The short pulse terminates before significant atomic (or electronic) motion commences, minimizing radiation damage. Simulated patterns for Photosystem I nanocrystals are used to develop a method for recovering structure factors from tens of thousands of snapshot patterns from nanocrystals varying in size, shape and orientation. We determine the number of shots needed for a required accuracy in structure factor measurement and resolution, and investigate the convergence of our Monte-Carlo integration method.
Original language | English (US) |
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Pages (from-to) | 5713-5723 |
Number of pages | 11 |
Journal | Optics Express |
Volume | 18 |
Issue number | 6 |
DOIs | |
State | Published - Mar 15 2010 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics