Fe-bleomycin as a probe of RNA conformation

Chris E. Holmes, Anil T. Abraham, Sidney M. Hecht, Catherine Florentz, Richard Giegé

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Two crystallographically defined tRNAs, yeast tRNA(Asp) and tRNA(Phe), were used as substrates for oxidative cleavage by Fe bleomycin to facilitate definition at high resolution of the structural elements in RNAs conducive to bleomycin binding and cleavage. Yeast tRNA(Asp) underwent cleavage at G45 and U66; yeast tRNA(Phe) was cleaved at four sites, namely G19, A31, U52 and A66. Only two of these six sites involved oxidative cleavage of a 5'-G·Pyr-3' sequence, but three sites were at the junction between single- and double-stranded regions of the RNA, consistent with a binding model:in which the bithiazole + C-terminal substituent of bleomycin bind to minor groove structures on the RNA. Also studied were four tRNA transcripts believed on the basis of biochemical and chemical mapping experiments to share structural elements in common with the mature tRNAs. Cleavage of these tRNAs by Fe bleomycin gave patterns of cleavage very different from each other and than those of the mature tRNAs. This observation suggests strongly that Fe bleomycin cannot be used for chemical mapping in the same fashion as more classical reagents, such as Pb2+ or dimethyl sulfate. However, the great sensitivity of Fe bleomycin to changes in nucleic acid structure argues that those species which do show similar patterns of cleavage must be very close in structure.

Original languageEnglish (US)
Pages (from-to)3399-3406
Number of pages8
JournalNucleic acids research
Volume24
Issue number17
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Holmes, C. E., Abraham, A. T., Hecht, S. M., Florentz, C., & Giegé, R. (1996). Fe-bleomycin as a probe of RNA conformation. Nucleic acids research, 24(17), 3399-3406. https://doi.org/10.1093/nar/24.17.3399