Fast separation of λ- and T2-DNA with electrodeless dielectrophoresis

J. Regtmeier, T. T. Duong, Alexandra Ros, D. Anselmetti

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The fast separation of long DNA via electrodeless dielectrophoresis in a simple and easy-to-fabricate microfluidic device is presented. An AC voltage is applied to an array of periodically arranged posts creating dielectrophoretic traps. Size-dependent migration velocities are obtained by applying an additional DC voltage, which drives the DNA through the structured microchannel. With this technique we are able to achieve fast and efficient separations of λ- and T2-DNA. The mechanism is analysed in terms of a Kramer's rate and a notable change in the diffusion coefficient of λ -DNA is observed.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages383-385
Number of pages3
StatePublished - 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

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Keywords

  • Dielectrophoresis
  • DNA
  • Microfluidics
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Regtmeier, J., Duong, T. T., Ros, A., & Anselmetti, D. (2006). Fast separation of λ- and T2-DNA with electrodeless dielectrophoresis. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 383-385). Japan Academic Association Inc.