Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels

Ruoming Peng, Chao Wang, Liangcheng Zhou, Qi Zhang, Weihua Zhang, Stephen Y. Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report fabrication and performances of a new immunoassay device, which integrates a novel nanoplasmonic sensor array inside a microfluidic channel. The new assay has demonstrated 106 fold detection limit enhancement of a model direct Protein A immunoassay over glass reference (from 2nM to 850 aM, i.e. 120 ng/ml to 50 fg/ml) and 6 fold of incubation time reduction (2 hours to 20 min) compared to conventional 96-well plate immunoassay.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages266-268
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Staphylococcal Protein A
Sensor arrays
Microfluidics
Assays
Demonstrations
Fluorescence
Proteins
Fabrication
Glass
Sensors

Keywords

  • And protein a
  • Fluorescence
  • Fluorescence enhancement
  • Immunoassay
  • Incubation time
  • Limit of detection
  • Microchannel
  • Microfluidics
  • Nanochannel
  • Nanofabrication
  • Nanoimprint
  • Nanoscale
  • Nanostructures
  • Plasmonics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Peng, R., Wang, C., Zhou, L., Zhang, Q., Zhang, W., & Chou, S. Y. (2012). Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 266-268). Chemical and Biological Microsystems Society.

Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. / Peng, Ruoming; Wang, Chao; Zhou, Liangcheng; Zhang, Qi; Zhang, Weihua; Chou, Stephen Y.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 266-268.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Peng, R, Wang, C, Zhou, L, Zhang, Q, Zhang, W & Chou, SY 2012, Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 266-268, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Peng R, Wang C, Zhou L, Zhang Q, Zhang W, Chou SY. Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 266-268
Peng, Ruoming ; Wang, Chao ; Zhou, Liangcheng ; Zhang, Qi ; Zhang, Weihua ; Chou, Stephen Y. / Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 266-268
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