Extraction and amplification of DNA from formalin-fixed, paraffin-embedded tissues

Lin Wu, Nancy Patten, Carl Yamashiro, Buena Chui

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Formalin-fixed, paraffin-embedded tissue (PET) is an invaluable resource for retrospective molecular genetic studies, but the extraction of high-quality genomic DNA from the PET may be problematic. We report a simple method that significantly improves the ability to amplify DNA recovered from formalin-fixed PET. Based on the standard procedure of a commercially available DNA preparation kit, the QIAamp DNA mini kit or the HighPure DNA preparation kit, we developed this method by eliminating the xylene/ethanol extraction step and adding a heat-treatment step. With this method, we have observed a five-to 10-fold increase in amplification efficiency of a fragment in a range of 90 to 386 base pairs. We also have obtained much higher amplification efficiencies for a multiplex polymerase chain reaction.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalApplied Immunohistochemistry and Molecular Morphology
Volume10
Issue number3
DOIs
StatePublished - Sep 1 2002
Externally publishedYes

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Paraffin
Formaldehyde
DNA
Xylenes
Multiplex Polymerase Chain Reaction
Base Pairing
Molecular Biology
Ethanol
Hot Temperature

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology

Cite this

Extraction and amplification of DNA from formalin-fixed, paraffin-embedded tissues. / Wu, Lin; Patten, Nancy; Yamashiro, Carl; Chui, Buena.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 10, No. 3, 01.09.2002, p. 269-274.

Research output: Contribution to journalArticle

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