TY - JOUR
T1 - Extracellular recording of spatiotemporal patterning in response to odors in the olfactory epithelium by microelectrode arrays
AU - Liu, Qingjun
AU - Hu, Ning
AU - Ye, Weiwei
AU - Cai, Hua
AU - Zhang, Fenni
AU - Wang, Ping
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 81071226 and 60725102), the Zhejiang Provincial Natural Science Foundation of China (No. Y2100684 and Y2111065), and the Scientific Research Fund of the Education Department of Zhejiang Province, China (No. Y200909323).
PY - 2011/9/15
Y1 - 2011/9/15
N2 - In olfactory biosensors, microelectronic sensor chips combined with biological olfactory cells are a promising platform for odor detection. In our investigation, olfactory epithelium stripped from rat was fixed on the surface of microelectrode arrays (MEAs). Electrophysiological activities of olfactory receptor neurons in intact epithelium were measured in the form of extracellular potentials. Based on multi-channel recording performance of MEA and structural and functional integrality of native olfactory epithelium, the spatiotemporal analysis was carried out to study the extracellular activity pattern of neurons in the tissue. The variation of spatiotemporal patterns corresponding to different odors displayed the signals firing image characteristic intuitionally. It is an effective method in the form of patterns for monitoring the state of tissue both in time and space domain, promoting the platform for olfactory sensing mechanism research.
AB - In olfactory biosensors, microelectronic sensor chips combined with biological olfactory cells are a promising platform for odor detection. In our investigation, olfactory epithelium stripped from rat was fixed on the surface of microelectrode arrays (MEAs). Electrophysiological activities of olfactory receptor neurons in intact epithelium were measured in the form of extracellular potentials. Based on multi-channel recording performance of MEA and structural and functional integrality of native olfactory epithelium, the spatiotemporal analysis was carried out to study the extracellular activity pattern of neurons in the tissue. The variation of spatiotemporal patterns corresponding to different odors displayed the signals firing image characteristic intuitionally. It is an effective method in the form of patterns for monitoring the state of tissue both in time and space domain, promoting the platform for olfactory sensing mechanism research.
KW - Cell based biosensor
KW - Microelectrode array (MEA)
KW - Olfactory epithelium
KW - Olfactory sensor
KW - Spatiotemporal patterning
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U2 - 10.1016/j.bios.2011.03.010
DO - 10.1016/j.bios.2011.03.010
M3 - Article
C2 - 21775126
AN - SCOPUS:79960911688
SN - 0956-5663
VL - 27
SP - 12
EP - 17
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -