External guide sequences for an RNA enzyme

Anthony C. Forster, Sidney Altman

Research output: Contribution to journalArticlepeer-review

254 Scopus citations


Ribonuclease P (RNase P) from Escherichia coli or its catalytic RNA subunit can efficiently cleave small RNA substrates that lack the conserved features of natural substrates of RNase P if an additional small RNA is also present. This additional RNA must contain a sequence complementary to the substrate [external guide sequence (EGS)] and a 3′-proximal CCA sequence to ensure cleavage. The aminoacyl acceptor stem and some additional 5′- and 3′-terminal sequences of a precursor transfer RNA are sufficient to allow efficient cleavage by RNase P, and the 2′-hydroxyl group at the cleavage site is not absolutely necessary for cleavage. In principle, any RNA could be targeted by a custom-designed EGS RNA for specific cleavage by RNase P in vitro or in vivo.

Original languageEnglish (US)
Pages (from-to)783-786
Number of pages4
Issue number4970
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'External guide sequences for an RNA enzyme'. Together they form a unique fingerprint.

Cite this