Extensive, recent lntron gains in Daphnia populations

Wenli Li, Abraham E. Tucker, Way Sung, W. Kelley Thomas, Michael Lynch

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Rates and mechanisms of intron gain and loss have traditionally been inferred from alignments of highly conserved genes sampled from phylogenetically distant taxa. We report a populationgenomic approach that detected 24 discordant intron/exon boundaries between the whole-genome sequences of two Daphnia pulex isolates. Sequencing of presence/absence loci across a collection of D. pulex isolates and outgroup Daphnia species shows that most polymorphisms are a consequence of recent gains, with parallel gains often occurring at the same locations in independent allelic lineages. More than half of the recent gains are associated with short sequence repeats, suggesting an origin via repair of staggered double-strand breaks. By comparing the allele-frequency spectrum of intron-gain alleles with that for derived single-base substitutions, we also provide evidence that newly arisen introns are intrinsically deleterious and tend to accumulate in population-genetic settings where random genetic drift is a relatively strong force.

Original languageEnglish (US)
Pages (from-to)1260-1262
Number of pages3
JournalScience
Volume326
Issue number5957
DOIs
StatePublished - Nov 27 2009

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Extensive, recent lntron gains in Daphnia populations'. Together they form a unique fingerprint.

  • Cite this

    Li, W., Tucker, A. E., Sung, W., Kelley Thomas, W., & Lynch, M. (2009). Extensive, recent lntron gains in Daphnia populations. Science, 326(5957), 1260-1262. https://doi.org/10.1126/science.1179302