Extensive neuroprotection by choroid plexus transplants in excitotoxin lesioned monkeys

Dwaine F. Emerich, Christopher G. Thanos, Moses Goddard, Stephen J.M. Skinner, Marilyn S. Geany, William J. Bell, Briannan Bintz, Patricia Schneider, Yaping Chu, Rangasamy Suresh Babu, Cesario V. Borlongan, Kim Boekelheide, Susan Hall, Bronwyn Bryant, Jeffrey H. Kordower

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Huntington's disease (HD) results from degeneration of striatal neurons. Choroid plexus (CP) cells secrete neurotrophic factors, and CP transplants are neuroprotective in rat models of HD. To determine if similar neuroprotective effects could be obtained in primates, porcine CP was encapsulated in alginate capsules. PCR confirmed that the CP cells expressed transthyretin and immunocytochemistry demonstrated typical ZO-1 and tubulin staining. In vitro, CP conditioned media enhanced the survival and preserved neurite number and length on serum deprived neurons. Cynomolgus primates were transplanted with CP-loaded capsules into the caudate and putamen followed by quinolinic acid (QA) lesions 1 week later. Control monkeys received empty capsules plus QA. Choroid plexus transplants significantly protected striatal neurons as revealed by stereological counts of NeuN-positive neurons (8% loss vs. 43% in controls) and striatum volume (10% decrease vs. 40% in controls). These data indicate that CP transplants might be useful for preventing the degeneration of neurons in HD.

Original languageEnglish (US)
Pages (from-to)471-480
Number of pages10
JournalNeurobiology of Disease
Volume23
Issue number2
DOIs
StatePublished - Aug 2006
Externally publishedYes

Keywords

  • Alginate
  • Choroid plexus
  • CNS transplantation
  • Encapsulation
  • Neuroprotection

ASJC Scopus subject areas

  • Neurology

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