Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants

Nicholas Renzette, Bornali Bhattacharjee, Jeffrey Jensen, Laura Gibson, Timothy F. Kowalik

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus.

Original languageEnglish (US)
Article numbere1001344
JournalPLoS Pathogens
Volume7
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

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Cytomegalovirus
RNA Viruses
Genome
Population
Viruses
DNA Viruses
Cytomegalovirus Infections
Population Genetics
Human Genome
Complex Mixtures
Nucleotides
HIV
Urine
Newborn Infant
RNA
Amino Acids
Research

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants. / Renzette, Nicholas; Bhattacharjee, Bornali; Jensen, Jeffrey; Gibson, Laura; Kowalik, Timothy F.

In: PLoS Pathogens, Vol. 7, No. 5, e1001344, 05.2011.

Research output: Contribution to journalArticle

Renzette, Nicholas ; Bhattacharjee, Bornali ; Jensen, Jeffrey ; Gibson, Laura ; Kowalik, Timothy F. / Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants. In: PLoS Pathogens. 2011 ; Vol. 7, No. 5.
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