Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

Mian Gao, Nicole Brufatto, Tricia Chen, Laura Lea Murley, Rosanne Thalakada, Megan Domagala, Bryan Beattie, Daniel Mamelak, Vicki Athanasopoulos, David Johnson, Douglas McFadden, Christian Burks, Lori Frappier

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.

Original languageEnglish (US)
Pages (from-to)2225-2235
Number of pages11
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Vaccinia virus
Baculoviridae
Herpesviridae
Viruses
Screening
Throughput
Solubility
Proteins
Distillation columns
Human Herpesvirus 1
Human Herpesvirus 4
Escherichia coli
Purification
Testing

Keywords

  • Baculovirus
  • Cytomegalovirus
  • Epstein-Barr virus
  • Herpes simplex virus
  • High-throughput protein expression
  • Vaccinia

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Gao, M., Brufatto, N., Chen, T., Murley, L. L., Thalakada, R., Domagala, M., ... Frappier, L. (2005). Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system. Journal of Proteome Research, 4(6), 2225-2235. https://doi.org/10.1021/pr050137u

Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system. / Gao, Mian; Brufatto, Nicole; Chen, Tricia; Murley, Laura Lea; Thalakada, Rosanne; Domagala, Megan; Beattie, Bryan; Mamelak, Daniel; Athanasopoulos, Vicki; Johnson, David; McFadden, Douglas; Burks, Christian; Frappier, Lori.

In: Journal of Proteome Research, Vol. 4, No. 6, 01.11.2005, p. 2225-2235.

Research output: Contribution to journalArticle

Gao, M, Brufatto, N, Chen, T, Murley, LL, Thalakada, R, Domagala, M, Beattie, B, Mamelak, D, Athanasopoulos, V, Johnson, D, McFadden, D, Burks, C & Frappier, L 2005, 'Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system', Journal of Proteome Research, vol. 4, no. 6, pp. 2225-2235. https://doi.org/10.1021/pr050137u
Gao, Mian ; Brufatto, Nicole ; Chen, Tricia ; Murley, Laura Lea ; Thalakada, Rosanne ; Domagala, Megan ; Beattie, Bryan ; Mamelak, Daniel ; Athanasopoulos, Vicki ; Johnson, David ; McFadden, Douglas ; Burks, Christian ; Frappier, Lori. / Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system. In: Journal of Proteome Research. 2005 ; Vol. 4, No. 6. pp. 2225-2235.
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