Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

Mian Gao, Nicole Brufatto, Tricia Chen, Laura Lea Murley, Rosanne Thalakada, Megan Domagala, Bryan Beattie, Daniel Mamelak, Vicki Athanasopoulos, David Johnson, Grant McFadden, Christian Burks, Lori Frappier

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.

Original languageEnglish (US)
Pages (from-to)2225-2235
Number of pages11
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2005

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Keywords

  • Baculovirus
  • Cytomegalovirus
  • Epstein-Barr virus
  • Herpes simplex virus
  • High-throughput protein expression
  • Vaccinia

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Gao, M., Brufatto, N., Chen, T., Murley, L. L., Thalakada, R., Domagala, M., Beattie, B., Mamelak, D., Athanasopoulos, V., Johnson, D., McFadden, G., Burks, C., & Frappier, L. (2005). Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system. Journal of Proteome Research, 4(6), 2225-2235. https://doi.org/10.1021/pr050137u