Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells

John J. Maurer, Teresa A. Doggett, Lisa Burns-Keliher, Roy Curtiss

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cells, by using bacterial luciferase as a reporter. We focused on gene expression controlled by Salmonella rfa promoter, using lac promoter as a control. We observed down regulation for both promoters during the initial 2 h of invasion. The decreased levels of luciferase activity appeared to be due to metabolic changes, since we observed similar results with tissue culture medium alone. Gene expression stabilized to a new steady state for the Salmonella rfa promoter, while a lac promoter activity steadily decreased. Bacterial luciferase activity was a good indicator of intracellular numbers and allowed us to detect as few as 1000 bacterial cells/infected monolayer. Both promoters were not dependent on host protein synthesis for expression.

Original languageEnglish (US)
Pages (from-to)172-176
Number of pages5
JournalCurrent Microbiology
Volume41
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Salmonella typhimurium
Bacterial Luciferases
Salmonella
Epithelial Cells
Gene Expression
Eukaryotic Cells
Luciferases
Culture Media
Down-Regulation
Genes
Proteins

ASJC Scopus subject areas

  • Microbiology

Cite this

Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells. / Maurer, John J.; Doggett, Teresa A.; Burns-Keliher, Lisa; Curtiss, Roy.

In: Current Microbiology, Vol. 41, No. 3, 2000, p. 172-176.

Research output: Contribution to journalArticle

Maurer, JJ, Doggett, TA, Burns-Keliher, L & Curtiss, R 2000, 'Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells', Current Microbiology, vol. 41, no. 3, pp. 172-176.
Maurer, John J. ; Doggett, Teresa A. ; Burns-Keliher, Lisa ; Curtiss, Roy. / Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells. In: Current Microbiology. 2000 ; Vol. 41, No. 3. pp. 172-176.
@article{1c077318d8b74c2ba26f844a98eedf5b,
title = "Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells",
abstract = "Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cells, by using bacterial luciferase as a reporter. We focused on gene expression controlled by Salmonella rfa promoter, using lac promoter as a control. We observed down regulation for both promoters during the initial 2 h of invasion. The decreased levels of luciferase activity appeared to be due to metabolic changes, since we observed similar results with tissue culture medium alone. Gene expression stabilized to a new steady state for the Salmonella rfa promoter, while a lac promoter activity steadily decreased. Bacterial luciferase activity was a good indicator of intracellular numbers and allowed us to detect as few as 1000 bacterial cells/infected monolayer. Both promoters were not dependent on host protein synthesis for expression.",
author = "Maurer, {John J.} and Doggett, {Teresa A.} and Lisa Burns-Keliher and Roy Curtiss",
year = "2000",
language = "English (US)",
volume = "41",
pages = "172--176",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells

AU - Maurer, John J.

AU - Doggett, Teresa A.

AU - Burns-Keliher, Lisa

AU - Curtiss, Roy

PY - 2000

Y1 - 2000

N2 - Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cells, by using bacterial luciferase as a reporter. We focused on gene expression controlled by Salmonella rfa promoter, using lac promoter as a control. We observed down regulation for both promoters during the initial 2 h of invasion. The decreased levels of luciferase activity appeared to be due to metabolic changes, since we observed similar results with tissue culture medium alone. Gene expression stabilized to a new steady state for the Salmonella rfa promoter, while a lac promoter activity steadily decreased. Bacterial luciferase activity was a good indicator of intracellular numbers and allowed us to detect as few as 1000 bacterial cells/infected monolayer. Both promoters were not dependent on host protein synthesis for expression.

AB - Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cells, by using bacterial luciferase as a reporter. We focused on gene expression controlled by Salmonella rfa promoter, using lac promoter as a control. We observed down regulation for both promoters during the initial 2 h of invasion. The decreased levels of luciferase activity appeared to be due to metabolic changes, since we observed similar results with tissue culture medium alone. Gene expression stabilized to a new steady state for the Salmonella rfa promoter, while a lac promoter activity steadily decreased. Bacterial luciferase activity was a good indicator of intracellular numbers and allowed us to detect as few as 1000 bacterial cells/infected monolayer. Both promoters were not dependent on host protein synthesis for expression.

UR - http://www.scopus.com/inward/record.url?scp=0033863993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033863993&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 172

EP - 176

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 3

ER -