Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii

Kiera Reifschneider-Wegner, Andrey Kanygin, Kevin Redding

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Biological hydrogen generation from phototrophic organisms is a promising source of renewable fuel. The nuclear-expressed [FeFe] hydrogenase from Chlamydomonas reinhardtii has an extremely high turnover rate, and so has been a target of intense research. Here, we demonstrate that a codon-optimized native hydrogenase can be successfully expressed in the chloroplast. We also demonstrate a curiously strong negative selective pressure resulting from unregulated hydrogenase expression in this location, and discuss management of its expression with a vitamin-controlled gene repression system. To the best of our knowledge, this represents the first example of a nuclear-expressed, chloroplast-localized metalloprotein being synthesized in situ. Control of this process opens up several bioengineering possibilities for the production of biohydrogen.

Original languageEnglish (US)
Pages (from-to)3657-3665
Number of pages9
JournalInternational Journal of Hydrogen Energy
Volume39
Issue number8
DOIs
StatePublished - Mar 6 2014

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chloroplasts
Vitamins
Genes
bioengineering
vitamins
Hydrogen
organisms
genes
hydrogen
Bioengineering
Metalloproteins

Keywords

  • [FeFe] hydrogenase
  • Chlamydomonas
  • Chloroplast
  • Gene expression
  • Green algae

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. / Reifschneider-Wegner, Kiera; Kanygin, Andrey; Redding, Kevin.

In: International Journal of Hydrogen Energy, Vol. 39, No. 8, 06.03.2014, p. 3657-3665.

Research output: Contribution to journalArticle

Reifschneider-Wegner, Kiera ; Kanygin, Andrey ; Redding, Kevin. / Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. In: International Journal of Hydrogen Energy. 2014 ; Vol. 39, No. 8. pp. 3657-3665.
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