Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii

Kiera Reifschneider-Wegner, Andrey Kanygin, Kevin Redding

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Biological hydrogen generation from phototrophic organisms is a promising source of renewable fuel. The nuclear-expressed [FeFe] hydrogenase from Chlamydomonas reinhardtii has an extremely high turnover rate, and so has been a target of intense research. Here, we demonstrate that a codon-optimized native hydrogenase can be successfully expressed in the chloroplast. We also demonstrate a curiously strong negative selective pressure resulting from unregulated hydrogenase expression in this location, and discuss management of its expression with a vitamin-controlled gene repression system. To the best of our knowledge, this represents the first example of a nuclear-expressed, chloroplast-localized metalloprotein being synthesized in situ. Control of this process opens up several bioengineering possibilities for the production of biohydrogen.

    Original languageEnglish (US)
    Pages (from-to)3657-3665
    Number of pages9
    JournalInternational Journal of Hydrogen Energy
    Volume39
    Issue number8
    DOIs
    StatePublished - Mar 6 2014

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    chloroplasts
    Vitamins
    Genes
    bioengineering
    vitamins
    Hydrogen
    organisms
    genes
    hydrogen
    Bioengineering
    Metalloproteins

    Keywords

    • [FeFe] hydrogenase
    • Chlamydomonas
    • Chloroplast
    • Gene expression
    • Green algae

    ASJC Scopus subject areas

    • Renewable Energy, Sustainability and the Environment
    • Fuel Technology
    • Condensed Matter Physics
    • Energy Engineering and Power Technology

    Cite this

    Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. / Reifschneider-Wegner, Kiera; Kanygin, Andrey; Redding, Kevin.

    In: International Journal of Hydrogen Energy, Vol. 39, No. 8, 06.03.2014, p. 3657-3665.

    Research output: Contribution to journalArticle

    Reifschneider-Wegner, Kiera ; Kanygin, Andrey ; Redding, Kevin. / Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. In: International Journal of Hydrogen Energy. 2014 ; Vol. 39, No. 8. pp. 3657-3665.
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