Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato

A. M. Walmsley, M. L. Alvarez, Y. Jin, D. D. Kirk, S. M. Lee, J. Pinkhasov, M. M. Rigano, C. J. Arntzen, Hugh Mason

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Epitopes often require co-delivery with an adjuvant or targeting protein to enable recognition by the immune system. This paper reports the ability of transgenic tomato plants to express a fusion protein consisting of the B subunit of the Escherichia coli heatlabile enterotoxin (LTB) and an immunocontraceptive epitope. The fusion protein was found to assemble into pentamers, as evidenced by its ability to bind to gangliosides, and had an average expression level of 37.8 μg g-1 in freeze-dried transgenic tissues. Processing of selected transgenic fruit resulted in a 16-fold increase in concentration of the antigen with minimal loss in detectable antigen. The species-specific nature of this epitope was shown by the inability of antibodies raised against non-target species to detect the LTB fusion protein. The immunocontraceptive ability of this vaccine will be tested in future pilot mice studies.

Original languageEnglish (US)
Pages (from-to)1020-1026
Number of pages7
JournalPlant Cell Reports
Volume21
Issue number10
DOIs
StatePublished - Jun 1 2003

Fingerprint

enterotoxins
genetically modified organisms
tomatoes
epitopes
Escherichia coli
heat
proteins
antigens
gangliosides
nontarget organisms
adjuvants
immune system
vaccines
antibodies
fruits
mice

Keywords

  • Fusion protein
  • Heat labile toxin
  • Immunocontraceptive
  • Tomato
  • Transgenic plants

ASJC Scopus subject areas

  • Plant Science

Cite this

Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato. / Walmsley, A. M.; Alvarez, M. L.; Jin, Y.; Kirk, D. D.; Lee, S. M.; Pinkhasov, J.; Rigano, M. M.; Arntzen, C. J.; Mason, Hugh.

In: Plant Cell Reports, Vol. 21, No. 10, 01.06.2003, p. 1020-1026.

Research output: Contribution to journalArticle

Walmsley, AM, Alvarez, ML, Jin, Y, Kirk, DD, Lee, SM, Pinkhasov, J, Rigano, MM, Arntzen, CJ & Mason, H 2003, 'Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato', Plant Cell Reports, vol. 21, no. 10, pp. 1020-1026. https://doi.org/10.1007/s00299-003-0619-4
Walmsley, A. M. ; Alvarez, M. L. ; Jin, Y. ; Kirk, D. D. ; Lee, S. M. ; Pinkhasov, J. ; Rigano, M. M. ; Arntzen, C. J. ; Mason, Hugh. / Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato. In: Plant Cell Reports. 2003 ; Vol. 21, No. 10. pp. 1020-1026.
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