Expression of recombinant human acetylcholinesterase in transgenic tomato plants

Tsafrir S. Mor; Meira Sternfeld; Hermona Soreq; Charles J. Arntzen; Hugh S. Mason

doi:10.1002/bit.10012

Expression of recombinant human acetylcholinesterase in transgenic tomato plants

Tsafrir S. Mor, Meira Sternfeld, Hermona Soreq, Charles J. Arntzen, Hugh S. Mason

Arizona State University

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min^-1 mg protein^-1 and fruits (up to 250 nmol min^-1 mg protein^-1).

Original language	English (US)
Pages (from-to)	259-266
Number of pages	8
Journal	Biotechnology and bioengineering
Volume	75
Issue number	3
DOIs	https://doi.org/10.1002/bit.10012
State	Published - Nov 5 2001

Keywords

(Acetyl)cholinesterase
Anticholinesterase(s)
Enzyme therapy
Nerve gas (protection)
Transgenic (plants)

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.10012

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title = "Expression of recombinant human acetylcholinesterase in transgenic tomato plants",

abstract = "Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min-1 mg protein-1 and fruits (up to 250 nmol min-1 mg protein-1).",

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AU - Mor, Tsafrir S.

AU - Sternfeld, Meira

AU - Soreq, Hermona

AU - Arntzen, Charles J.

AU - Mason, Hugh S.

PY - 2001/11/5

Y1 - 2001/11/5

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AB - Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min-1 mg protein-1 and fruits (up to 250 nmol min-1 mg protein-1).

KW - (Acetyl)cholinesterase

KW - Anticholinesterase(s)

KW - Enzyme therapy

KW - Nerve gas (protection)

KW - Transgenic (plants)

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Expression of recombinant human acetylcholinesterase in transgenic tomato plants

Abstract

Keywords

ASJC Scopus subject areas

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