Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia

Suzanne M. Gignac, Michael Buschle, George Pettit, A. V. Hoffbrand, Hans G. Drexler

Research output: Contribution to journalArticle

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Abstract

We describe in vitro studies undertaken to characterize the expression of the proto-oncogene c-jun during differentiation of B-CLL cells. The phorbol ester TPA and the natural compound Bryostatin 1 (Bryo) were used to directly stimulate protein kinase C (PKC) while the calcium ionophone A23187 was employed to increase intracellular Ca2+. In quiescent cells c-jun mRNA expression was undetectable or at low levels. Upon treatment with TPA or Bryo, the steady-state levels of c-jun mRNA increased rapidly, reached a maximum at 0.5 or 1 hr, and then decreased in the B-CLL cells from all five patients analyzed; this reaction was augmented by the addition of A23187. induction of c-jun mRNA by direct stimulation of PKC could be blocked by the PKC inhibitor H7. The present observations, along with other results on the induction of long-term phenotypical cellular changes, such as alteration of morphology and other features of differentiation, support the notion that the second messenger (via PKC) and the third messenger (via proto-oncogene products) pathways are intact in B-chronic lymphocytic leukemia cells.

Original languageEnglish (US)
Pages (from-to)441-444
Number of pages4
JournalLeukemia
Volume4
Issue number6
StatePublished - Jun 1990

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jun Genes
B-Cell Chronic Lymphocytic Leukemia
Protein Kinase C
Calcimycin
Messenger RNA
Proto-Oncogenes
Protein C Inhibitor
Oncogene Proteins
Second Messenger Systems
Phorbol Esters
Protein Kinase Inhibitors
Calcium

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Gignac, S. M., Buschle, M., Pettit, G., Hoffbrand, A. V., & Drexler, H. G. (1990). Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia. Leukemia, 4(6), 441-444.

Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia. / Gignac, Suzanne M.; Buschle, Michael; Pettit, George; Hoffbrand, A. V.; Drexler, Hans G.

In: Leukemia, Vol. 4, No. 6, 06.1990, p. 441-444.

Research output: Contribution to journalArticle

Gignac, SM, Buschle, M, Pettit, G, Hoffbrand, AV & Drexler, HG 1990, 'Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia', Leukemia, vol. 4, no. 6, pp. 441-444.
Gignac SM, Buschle M, Pettit G, Hoffbrand AV, Drexler HG. Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia. Leukemia. 1990 Jun;4(6):441-444.
Gignac, Suzanne M. ; Buschle, Michael ; Pettit, George ; Hoffbrand, A. V. ; Drexler, Hans G. / Expression of proto-oncogene c-jun during differentiation of B-chronic lymphocytic leukemia. In: Leukemia. 1990 ; Vol. 4, No. 6. pp. 441-444.
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