Expression of porcine prorelaxin in transgenic tobacco

Scott Buswell, Fabricio Medina-Bolivar, Qiang Chen, Kevin Van Cott, Chenming Zhang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In vitro studies demonstrate that porcine relaxin may possess various therapeutic effects. In this study, we explore the possibility of expressing porcine relaxin in transgenic tobacco. Tobacco was selected because it is a non-food, non-feed crop, and recombinant protein production can readily be scaled up. The cDNA of porcine preprorelaxin was under the regulation of two different constitutive promoters. DNA analysis by polymerase chain reaction verified that all transgenic plants contained the correct size of gene insert. Preliminary studies showed the presence of putative prorelaxin hands in both silver-stained SDS-PAGE and western blot. The results also indicated that tobacco-produced prorelaxin may not be properly processed to yield the mature relaxin.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalAnnals of the New York Academy of Sciences
Volume1041
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Relaxin
Tobacco
Swine
Genetically Modified Plants
Polymerase chain reaction
Therapeutic Uses
DNA-Directed DNA Polymerase
Silver
Recombinant Proteins
Crops
Polyacrylamide Gel Electrophoresis
Hand
Complementary DNA
Genes
Western Blotting
Polymerase Chain Reaction
prorelaxin

Keywords

  • Porcine relaxin
  • Recombinant protein
  • Relaxin
  • Transgenic plants
  • Transgenic tobacco

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Expression of porcine prorelaxin in transgenic tobacco. / Buswell, Scott; Medina-Bolivar, Fabricio; Chen, Qiang; Van Cott, Kevin; Zhang, Chenming.

In: Annals of the New York Academy of Sciences, Vol. 1041, 2005, p. 77-81.

Research output: Contribution to journalArticle

Buswell, Scott ; Medina-Bolivar, Fabricio ; Chen, Qiang ; Van Cott, Kevin ; Zhang, Chenming. / Expression of porcine prorelaxin in transgenic tobacco. In: Annals of the New York Academy of Sciences. 2005 ; Vol. 1041. pp. 77-81.
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