Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought- and salt tolerance and increases fibre yield in the field conditions

Vijaya Pasapula, Guoxin Shen, Sundaram Kuppu, Julio Paez-Valencia, Marisol Mendoza, Pei Hou, Jian Chen, Xiaoyun Qiu, Longfu Zhu, Xianlong Zhang, Dick Auld, Eduardo Blumwald, Hong Zhang, Roberto Gaxiola, Paxton Payton

Research output: Contribution to journalArticle

170 Scopus citations

Abstract

The Arabidopsis gene AVP1 encodes a vacuolar pyrophosphatase that functions as a proton pump on the vacuolar membrane. Overexpression of AVP1 in Arabidopsis, tomato and rice enhances plant performance under salt and drought stress conditions, because up-regulation of the type I H+-PPase from Arabidopsis may result in a higher proton electrochemical gradient, which facilitates enhanced sequestering of ions and sugars into the vacuole, reducing water potential and resulting in increased drought- and salt tolerance when compared to wild-type plants. Furthermore, overexpression of AVP1 stimulates auxin transport in the root system and leads to larger root systems, which helps transgenic plants absorb water more efficiently under drought conditions. Using the same approach, AVP1-expressing cotton plants were created and tested for their performance under high-salt and reduced irrigation conditions. The AVP1-expressing cotton plants showed more vigorous growth than wild-type plants in the presence of 200-mm NaCl under hydroponic growth conditions. The soil-grown AVP1-expressing cotton plants also displayed significantly improved tolerance to both drought and salt stresses in greenhouse conditions. Furthermore, the fibre yield of AVP1-expressing cotton plants is at least 20% higher than that of wild-type plants under dry-land conditions in the field. This research indicates that AVP1 has the potential to be used for improving crop's drought- and salt tolerance in areas where water and salinity are limiting factors for agricultural productivity. Plant Biotechnology Journal

Original languageEnglish (US)
Pages (from-to)88-99
Number of pages12
JournalPlant Biotechnology Journal
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2011

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Keywords

  • Drought tolerance
  • Proton pump
  • Root development
  • Salt stress
  • Transgenic cotton

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Pasapula, V., Shen, G., Kuppu, S., Paez-Valencia, J., Mendoza, M., Hou, P., Chen, J., Qiu, X., Zhu, L., Zhang, X., Auld, D., Blumwald, E., Zhang, H., Gaxiola, R., & Payton, P. (2011). Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought- and salt tolerance and increases fibre yield in the field conditions. Plant Biotechnology Journal, 9(1), 88-99. https://doi.org/10.1111/j.1467-7652.2010.00535.x