Expression and evolutionary analysis of West Nile virus (Merion Strain)

Mathura P. Ramanathan, Jerome A. Chambers, Jesse Taylor, Bette T. Korber, Mark D. Lee, Aysegul Nalca, Kesan Dang, Panyupa Pankhong, Watcharee Attatippaholkun, David B. Weiner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The authors report a new strain of West Nile virus (WNV) with the expression analysis of its individual open reading frames. Since its sudden appearance in the summer of 1999 in New York City, the virus has spread rapidly across the continental United States into Canada and Mexico. Besides, its rapid transmission by various vectors, the spread of this virus through organ transplantation, blood transfusion, and mother-child transmission through breast milk is of concern. In order to understand molecular variations of WNV in North America and to generate new tools for understanding WNV biology, a complete clone of WNV has been constructed. Investigations so far have focused only on half of its genes products and a detailed molecular and cell biological aspects on all of WNV gene have yet to be clearly established. The open reading frames of WNV were recovered through an reverse transcriptase-polymerase chain reaction (RT-PCR)-PCR using brain tissue from a dead crow collected in Merion, PA, and cloned into a mammalian expression vector. The deduced amino acid sequences of individual open reading frames were analyzed to determine various structural motifs and functional domains. Expression analysis shows that in neuronal cells, C, NS1, and NS5 proteins are nuclear localized whereas the rest of the antigens are confined to the cytoplasm when they are expressed in the absence of other viral antigens. This is the first report that provides an expression analysis as well as intracellular distribution pattern for all of WNV gene products, cloned from an infected bird. Evolutionary analysis of Merion strain sequences indicates that this strain is distinct phylogenetically from the previously reported WNV strains.

Original languageEnglish (US)
Pages (from-to)544-556
Number of pages13
JournalJournal of NeuroVirology
Volume11
Issue number6
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

Fingerprint

West Nile virus
Open Reading Frames
Genes
Viruses
Crows
Viral Antigens
Organ Transplantation
Human Milk
Nuclear Proteins
North America
Mexico
Reverse Transcriptase Polymerase Chain Reaction
Blood Transfusion
Canada
Birds
Amino Acid Sequence
Cytoplasm
Clone Cells
Mothers
Antigens

Keywords

  • Merion strain
  • Phylogenetic analysis
  • West Nile virus
  • WNV antigens
  • WNV pathogenesis

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

Cite this

Ramanathan, M. P., Chambers, J. A., Taylor, J., Korber, B. T., Lee, M. D., Nalca, A., ... Weiner, D. B. (2005). Expression and evolutionary analysis of West Nile virus (Merion Strain). Journal of NeuroVirology, 11(6), 544-556. https://doi.org/10.1080/13550280500385229

Expression and evolutionary analysis of West Nile virus (Merion Strain). / Ramanathan, Mathura P.; Chambers, Jerome A.; Taylor, Jesse; Korber, Bette T.; Lee, Mark D.; Nalca, Aysegul; Dang, Kesan; Pankhong, Panyupa; Attatippaholkun, Watcharee; Weiner, David B.

In: Journal of NeuroVirology, Vol. 11, No. 6, 01.12.2005, p. 544-556.

Research output: Contribution to journalArticle

Ramanathan, MP, Chambers, JA, Taylor, J, Korber, BT, Lee, MD, Nalca, A, Dang, K, Pankhong, P, Attatippaholkun, W & Weiner, DB 2005, 'Expression and evolutionary analysis of West Nile virus (Merion Strain)', Journal of NeuroVirology, vol. 11, no. 6, pp. 544-556. https://doi.org/10.1080/13550280500385229
Ramanathan, Mathura P. ; Chambers, Jerome A. ; Taylor, Jesse ; Korber, Bette T. ; Lee, Mark D. ; Nalca, Aysegul ; Dang, Kesan ; Pankhong, Panyupa ; Attatippaholkun, Watcharee ; Weiner, David B. / Expression and evolutionary analysis of West Nile virus (Merion Strain). In: Journal of NeuroVirology. 2005 ; Vol. 11, No. 6. pp. 544-556.
@article{dbc3855a1c0f49149764bd1436c72dd5,
title = "Expression and evolutionary analysis of West Nile virus (Merion Strain)",
abstract = "The authors report a new strain of West Nile virus (WNV) with the expression analysis of its individual open reading frames. Since its sudden appearance in the summer of 1999 in New York City, the virus has spread rapidly across the continental United States into Canada and Mexico. Besides, its rapid transmission by various vectors, the spread of this virus through organ transplantation, blood transfusion, and mother-child transmission through breast milk is of concern. In order to understand molecular variations of WNV in North America and to generate new tools for understanding WNV biology, a complete clone of WNV has been constructed. Investigations so far have focused only on half of its genes products and a detailed molecular and cell biological aspects on all of WNV gene have yet to be clearly established. The open reading frames of WNV were recovered through an reverse transcriptase-polymerase chain reaction (RT-PCR)-PCR using brain tissue from a dead crow collected in Merion, PA, and cloned into a mammalian expression vector. The deduced amino acid sequences of individual open reading frames were analyzed to determine various structural motifs and functional domains. Expression analysis shows that in neuronal cells, C, NS1, and NS5 proteins are nuclear localized whereas the rest of the antigens are confined to the cytoplasm when they are expressed in the absence of other viral antigens. This is the first report that provides an expression analysis as well as intracellular distribution pattern for all of WNV gene products, cloned from an infected bird. Evolutionary analysis of Merion strain sequences indicates that this strain is distinct phylogenetically from the previously reported WNV strains.",
keywords = "Merion strain, Phylogenetic analysis, West Nile virus, WNV antigens, WNV pathogenesis",
author = "Ramanathan, {Mathura P.} and Chambers, {Jerome A.} and Jesse Taylor and Korber, {Bette T.} and Lee, {Mark D.} and Aysegul Nalca and Kesan Dang and Panyupa Pankhong and Watcharee Attatippaholkun and Weiner, {David B.}",
year = "2005",
month = "12",
day = "1",
doi = "10.1080/13550280500385229",
language = "English (US)",
volume = "11",
pages = "544--556",
journal = "Journal of NeuroVirology",
issn = "1355-0284",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Expression and evolutionary analysis of West Nile virus (Merion Strain)

AU - Ramanathan, Mathura P.

AU - Chambers, Jerome A.

AU - Taylor, Jesse

AU - Korber, Bette T.

AU - Lee, Mark D.

AU - Nalca, Aysegul

AU - Dang, Kesan

AU - Pankhong, Panyupa

AU - Attatippaholkun, Watcharee

AU - Weiner, David B.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The authors report a new strain of West Nile virus (WNV) with the expression analysis of its individual open reading frames. Since its sudden appearance in the summer of 1999 in New York City, the virus has spread rapidly across the continental United States into Canada and Mexico. Besides, its rapid transmission by various vectors, the spread of this virus through organ transplantation, blood transfusion, and mother-child transmission through breast milk is of concern. In order to understand molecular variations of WNV in North America and to generate new tools for understanding WNV biology, a complete clone of WNV has been constructed. Investigations so far have focused only on half of its genes products and a detailed molecular and cell biological aspects on all of WNV gene have yet to be clearly established. The open reading frames of WNV were recovered through an reverse transcriptase-polymerase chain reaction (RT-PCR)-PCR using brain tissue from a dead crow collected in Merion, PA, and cloned into a mammalian expression vector. The deduced amino acid sequences of individual open reading frames were analyzed to determine various structural motifs and functional domains. Expression analysis shows that in neuronal cells, C, NS1, and NS5 proteins are nuclear localized whereas the rest of the antigens are confined to the cytoplasm when they are expressed in the absence of other viral antigens. This is the first report that provides an expression analysis as well as intracellular distribution pattern for all of WNV gene products, cloned from an infected bird. Evolutionary analysis of Merion strain sequences indicates that this strain is distinct phylogenetically from the previously reported WNV strains.

AB - The authors report a new strain of West Nile virus (WNV) with the expression analysis of its individual open reading frames. Since its sudden appearance in the summer of 1999 in New York City, the virus has spread rapidly across the continental United States into Canada and Mexico. Besides, its rapid transmission by various vectors, the spread of this virus through organ transplantation, blood transfusion, and mother-child transmission through breast milk is of concern. In order to understand molecular variations of WNV in North America and to generate new tools for understanding WNV biology, a complete clone of WNV has been constructed. Investigations so far have focused only on half of its genes products and a detailed molecular and cell biological aspects on all of WNV gene have yet to be clearly established. The open reading frames of WNV were recovered through an reverse transcriptase-polymerase chain reaction (RT-PCR)-PCR using brain tissue from a dead crow collected in Merion, PA, and cloned into a mammalian expression vector. The deduced amino acid sequences of individual open reading frames were analyzed to determine various structural motifs and functional domains. Expression analysis shows that in neuronal cells, C, NS1, and NS5 proteins are nuclear localized whereas the rest of the antigens are confined to the cytoplasm when they are expressed in the absence of other viral antigens. This is the first report that provides an expression analysis as well as intracellular distribution pattern for all of WNV gene products, cloned from an infected bird. Evolutionary analysis of Merion strain sequences indicates that this strain is distinct phylogenetically from the previously reported WNV strains.

KW - Merion strain

KW - Phylogenetic analysis

KW - West Nile virus

KW - WNV antigens

KW - WNV pathogenesis

UR - http://www.scopus.com/inward/record.url?scp=31144450049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31144450049&partnerID=8YFLogxK

U2 - 10.1080/13550280500385229

DO - 10.1080/13550280500385229

M3 - Article

VL - 11

SP - 544

EP - 556

JO - Journal of NeuroVirology

JF - Journal of NeuroVirology

SN - 1355-0284

IS - 6

ER -