TY - JOUR
T1 - Expression analysis of neuroleukin, calmodulin, cortactin, and Rho7/Rnd2 in the intact and injured mouse brain
AU - Decourt, Boris
AU - Bouleau, Yohan
AU - Dulon, Didier
AU - Hafidi, Aziz
N1 - Funding Information:
This study was supported by Association Libre pour la Recherche sur la Moelle Epinière (ALARME). We would like to thank E. Normand and D. Bouchet (CNRS 5091, Bordeaux) for their technical help regarding the in situ hybridization and Western blot experiments, Dr. A. Raz for providing us with the anti-neuroleukin antibody, and Dr. James Saunders for helpful comments on the manuscript.
PY - 2005/9/8
Y1 - 2005/9/8
N2 - Subtracted cDNA libraries from the mouse developing inferior colliculus were previously constructed between postnatal day (P) 6 and 10. In the P10-P6 subtracted library, neuroleukin, calmodulin I, cortactin, and Rho7 were identified. The goal of the present study was to analyze their distribution, at the mRNA and protein levels, in both the adult and the developing mouse brain. The four molecules showed a wide expression throughout the brain, with a neuronal-enriched localization in structures such as the cortex, the hippocampus, the cerebellum, and the inferior colliculus. The level of expression of their corresponding mRNAs increased during brain postnatal development. The expression of these molecules was also investigated 2 weeks after a mechanical lesion in the adult cerebral cortex. Neuroleukin and cortactin were found to be expressed by reactive astrocytes, while there were no changes in the expression of calmodulin and Rho7. The expression of neuroleukin, calmodulin, cortactin, and Rho7 is discussed in the context of their putative role in the maturation of the brain and in the axonal regeneration process.
AB - Subtracted cDNA libraries from the mouse developing inferior colliculus were previously constructed between postnatal day (P) 6 and 10. In the P10-P6 subtracted library, neuroleukin, calmodulin I, cortactin, and Rho7 were identified. The goal of the present study was to analyze their distribution, at the mRNA and protein levels, in both the adult and the developing mouse brain. The four molecules showed a wide expression throughout the brain, with a neuronal-enriched localization in structures such as the cortex, the hippocampus, the cerebellum, and the inferior colliculus. The level of expression of their corresponding mRNAs increased during brain postnatal development. The expression of these molecules was also investigated 2 weeks after a mechanical lesion in the adult cerebral cortex. Neuroleukin and cortactin were found to be expressed by reactive astrocytes, while there were no changes in the expression of calmodulin and Rho7. The expression of neuroleukin, calmodulin, cortactin, and Rho7 is discussed in the context of their putative role in the maturation of the brain and in the axonal regeneration process.
KW - Astrocyte
KW - Axonal regeneration
KW - Development
KW - Inferior colliculus
KW - Injury
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U2 - 10.1016/j.devbrainres.2005.06.013
DO - 10.1016/j.devbrainres.2005.06.013
M3 - Article
C2 - 16051374
AN - SCOPUS:27644504936
SN - 0165-3806
VL - 159
SP - 36
EP - 54
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 1
ER -