Abstract

A method is presented for screening high-density arrays to discover peptides that bind and modulate enzyme activity. A polyvinyl alcohol solution was applied to array surfaces to limit the diffusion of product molecules released from enzymatic reactions, allowing the simultaneous measurement of enzyme activity and binding at each peptide spot. For proof of concept, it was possible to identify peptides that bound to horseradish peroxidase, alkaline phosphatase, and β-galactosidase and substantially altered enzyme activity by comparing the binding level of peptide to enzyme and bound enzyme activity. This basic technique may be generally applicable to find peptides or other small molecules that modify enzyme activity.

Original languageEnglish (US)
Pages (from-to)6419-6424
Number of pages6
JournalJournal of the American Chemical Society
Volume132
Issue number18
DOIs
StatePublished - May 12 2010

Fingerprint

Enzyme activity
Modulators
Peptides
Enzymes
Galactosidases
Polyvinyl Alcohol
Molecules
Polyvinyl alcohols
Phosphatases
Horseradish Peroxidase
Alkaline Phosphatase
Screening

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Exploring peptide space for enzyme modulators. / Fu, Jinglin; Cai, Katherine; Johnston, Stephen; Woodbury, Neal.

In: Journal of the American Chemical Society, Vol. 132, No. 18, 12.05.2010, p. 6419-6424.

Research output: Contribution to journalArticle

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