Exploring different strategies to express Dengue virus envelope protein in a plant system

Carolina Andrea Martínez, Emel Topal, Ana María Giulietti, Julián Rodríguez Talou, Hugh Mason

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.

Original languageEnglish (US)
Pages (from-to)867-875
Number of pages9
JournalBiotechnology Letters
Volume32
Issue number6
DOIs
StatePublished - Mar 10 2010

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Keywords

  • Dengue virus
  • Envelope protein
  • Magnifection
  • Nicotiana benthamiana
  • Transient expression

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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