TY - JOUR
T1 - Exploring different strategies to express Dengue virus envelope protein in a plant system
AU - Martínez, Carolina Andrea
AU - Topal, Emel
AU - Giulietti, Ana María
AU - Talou, Julián Rodríguez
AU - Mason, Hugh
N1 - Funding Information:
Acknowledgements CAM is supported by University of Buenos Aires. AMG and JRT are supported by CONICET (National Council of Scientific and Technical Research). CAM, AMG and JRT thank Dr Andrea Gamarnik for her help during the initial stages of this work and Juan A. Mondotte for his assistance. We wish to especially thank Dr Charles J. Arntzen for the opportunity to use the facilities of CIDV (Center for Infectious Diseases and Vaccinology) at the Biodesign Institute to do this study.
PY - 2010
Y1 - 2010
N2 - Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.
AB - Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.
KW - Dengue virus
KW - Envelope protein
KW - Magnifection
KW - Nicotiana benthamiana
KW - Transient expression
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U2 - 10.1007/s10529-010-0236-6
DO - 10.1007/s10529-010-0236-6
M3 - Article
C2 - 20213522
AN - SCOPUS:77952239645
SN - 0141-5492
VL - 32
SP - 867
EP - 875
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 6
ER -