Exploring different strategies to express Dengue virus envelope protein in a plant system

Carolina Andrea Martínez, Emel Topal, Ana María Giulietti, Julián Rodríguez Talou, Hugh Mason

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.

Original languageEnglish (US)
Pages (from-to)867-875
Number of pages9
JournalBiotechnology Letters
Volume32
Issue number6
DOIs
StatePublished - 2010

Fingerprint

Viral Envelope Proteins
Glycoproteins
Dengue Virus
Antigens
Viruses
Hepatitis B Core Antigens
Proteins
Antibodies
Viral Structural Proteins
Hepatitis B Antibodies
Recombinant proteins
Subunit Vaccines
Vaccines
Recombinant Proteins
Tobacco
Anti-Idiotypic Antibodies
Immunity
Fusion reactions

Keywords

  • Dengue virus
  • Envelope protein
  • Magnifection
  • Nicotiana benthamiana
  • Transient expression

ASJC Scopus subject areas

  • Biotechnology

Cite this

Exploring different strategies to express Dengue virus envelope protein in a plant system. / Martínez, Carolina Andrea; Topal, Emel; Giulietti, Ana María; Talou, Julián Rodríguez; Mason, Hugh.

In: Biotechnology Letters, Vol. 32, No. 6, 2010, p. 867-875.

Research output: Contribution to journalArticle

Martínez, Carolina Andrea ; Topal, Emel ; Giulietti, Ana María ; Talou, Julián Rodríguez ; Mason, Hugh. / Exploring different strategies to express Dengue virus envelope protein in a plant system. In: Biotechnology Letters. 2010 ; Vol. 32, No. 6. pp. 867-875.
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