Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale

Julien E. Gautrot, Britta Trappmann, Fabian Oceguera-Yanez, John Connelly, Ximin He, Fiona M. Watt, Wilhelm T S Huck

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The control of the cell microenvironment on model patterned substrates allows the systematic study of cell biology in well defined conditions, potentially using automated systems. The extreme protein resistance of poly(oligo(ethylene glycol methacrylate)) (POEGMA) brushes is exploited to achieve high fidelity patterning of single cells. These coatings can be patterned by soft lithography on large areas (a microscope slide) and scale (substrates were typically prepared in batches of 200). The present protocol relies on the adsorption of extra-cellular matrix (ECM) proteins on unprotected areas using simple incubation and washing steps. The stability of POEGMA brushes, as examined via ellipsometry and SPR, is found to be excellent, both during storage and cell culture. The impact of substrate treatment, brush thickness and incubation protocol on ECM deposition, both for ultra-thin gold and glass substrates, is investigated via fluorescence microscopy and AFM. Optimised conditions result in high quality ECM patterns at the micron scale, even on glass substrates, that are suitable for controlling cell spreading and polarisation. These patterns are compatible with state-of-the-art technologies (fluorescence microscopy, FRET) used for live cell imaging. This technology, combined with single cell analysis methods, provides a platform for exploring the mechanisms that regulate cell behaviour.

Original languageEnglish (US)
Pages (from-to)5030-5041
Number of pages12
JournalBiomaterials
Volume31
Issue number18
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Cell adhesion
Brushes
Cell Adhesion
Polymers
Polarization
Proteins
Substrates
Fluorescence Microscopy
Fluorescence microscopy
Glass
Polyethylene glycols
Single-Cell Analysis
Technology
Cellular Microenvironment
Cytology
Gold
Adsorption
Cell Biology
Ellipsometry
Cell culture

Keywords

  • Cell polarisation
  • Extra-cellular matrix
  • Patterning
  • Polymer brush
  • Single cell

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Gautrot, J. E., Trappmann, B., Oceguera-Yanez, F., Connelly, J., He, X., Watt, F. M., & Huck, W. T. S. (2010). Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale. Biomaterials, 31(18), 5030-5041. https://doi.org/10.1016/j.biomaterials.2010.02.066

Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale. / Gautrot, Julien E.; Trappmann, Britta; Oceguera-Yanez, Fabian; Connelly, John; He, Ximin; Watt, Fiona M.; Huck, Wilhelm T S.

In: Biomaterials, Vol. 31, No. 18, 06.2010, p. 5030-5041.

Research output: Contribution to journalArticle

Gautrot, JE, Trappmann, B, Oceguera-Yanez, F, Connelly, J, He, X, Watt, FM & Huck, WTS 2010, 'Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale', Biomaterials, vol. 31, no. 18, pp. 5030-5041. https://doi.org/10.1016/j.biomaterials.2010.02.066
Gautrot, Julien E. ; Trappmann, Britta ; Oceguera-Yanez, Fabian ; Connelly, John ; He, Ximin ; Watt, Fiona M. ; Huck, Wilhelm T S. / Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale. In: Biomaterials. 2010 ; Vol. 31, No. 18. pp. 5030-5041.
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