Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation

Jinghui Luo, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The study of mitochondria is important due to the organelle's contribution to many cellular functions, including metabolism, cellular signaling and apoptosis. The atypically sized giant mitochondria1 were found in the models of aging and some diseases. Thus it would be advantageous for related studies if mitochondrial subpopulations could be prepared in accordance with their particular sizes. Conventional methods such as centrifugation and electrophoresis can separate mitochondria from cell cytoplasm and further purify them from other organelles. However, the separation of mitochondrial subpopulations has rarely been studied. Here, we present the numerical simulation with the design of a microfluidic device to elucidate absolute negative mobility (ANM) utilizing insulator-based dielectrophoresis (iDEP) for the separation of mitochondrial subpopulations.

Original languageEnglish (US)
Title of host publicationAnnual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting
PublisherAIChE
Pages65-66
Number of pages2
ISBN (Electronic)9781510812437
StatePublished - 2014
EventAnnual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting - Atlanta, United States
Duration: Nov 16 2014Nov 21 2014

Other

OtherAnnual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting
CountryUnited States
CityAtlanta
Period11/16/1411/21/14

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Luo, J., & Ros, A. (2014). Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation. In Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting (pp. 65-66). AIChE.