Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation

Jinghui Luo; Alexandra Ros

Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation

Molecular Sciences, School of (SMS)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The study of mitochondria is important due to the organelle's contribution to many cellular functions, including metabolism, cellular signaling and apoptosis. The atypically sized giant mitochondria¹ were found in the models of aging and some diseases. Thus it would be advantageous for related studies if mitochondrial subpopulations could be prepared in accordance with their particular sizes. Conventional methods such as centrifugation and electrophoresis can separate mitochondria from cell cytoplasm and further purify them from other organelles. However, the separation of mitochondrial subpopulations has rarely been studied. Here, we present the numerical simulation with the design of a microfluidic device to elucidate absolute negative mobility (ANM) utilizing insulator-based dielectrophoresis (iDEP) for the separation of mitochondrial subpopulations.

Original language	English (US)
Title of host publication	Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting
Publisher	AIChE
Pages	65-66
Number of pages	2
ISBN (Electronic)	9781510812437
State	Published - 2014
Event	Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting - Atlanta, United States Duration: Nov 16 2014 → Nov 21 2014

Other

Other	Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting
Country/Territory	United States
City	Atlanta
Period	11/16/14 → 11/21/14

ASJC Scopus subject areas

Electrical and Electronic Engineering

Cite this

Luo, J & Ros, A 2014, Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation. in Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting. AIChE, pp. 65-66, Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting, Atlanta, United States, 11/16/14.

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abstract = "The study of mitochondria is important due to the organelle's contribution to many cellular functions, including metabolism, cellular signaling and apoptosis. The atypically sized giant mitochondria1 were found in the models of aging and some diseases. Thus it would be advantageous for related studies if mitochondrial subpopulations could be prepared in accordance with their particular sizes. Conventional methods such as centrifugation and electrophoresis can separate mitochondria from cell cytoplasm and further purify them from other organelles. However, the separation of mitochondrial subpopulations has rarely been studied. Here, we present the numerical simulation with the design of a microfluidic device to elucidate absolute negative mobility (ANM) utilizing insulator-based dielectrophoresis (iDEP) for the separation of mitochondrial subpopulations.",

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booktitle = "Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting",

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AU - Ros, Alexandra

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AB - The study of mitochondria is important due to the organelle's contribution to many cellular functions, including metabolism, cellular signaling and apoptosis. The atypically sized giant mitochondria1 were found in the models of aging and some diseases. Thus it would be advantageous for related studies if mitochondrial subpopulations could be prepared in accordance with their particular sizes. Conventional methods such as centrifugation and electrophoresis can separate mitochondria from cell cytoplasm and further purify them from other organelles. However, the separation of mitochondrial subpopulations has rarely been studied. Here, we present the numerical simulation with the design of a microfluidic device to elucidate absolute negative mobility (ANM) utilizing insulator-based dielectrophoresis (iDEP) for the separation of mitochondrial subpopulations.

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M3 - Conference contribution

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BT - Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting

PB - AIChE

T2 - Annual Meeting of the American Electrophoresis Society 2014, AES 2014 - Topical Conference at the 2014 AIChE Annual Meeting

Y2 - 16 November 2014 through 21 November 2014

ER -

Exploiting absolute negative mobility with dielectrophoresis for mitochondrial sample preparation

Abstract

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ASJC Scopus subject areas

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