Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses

Benedikt J. Daurer, Kenta Okamoto, Johan Bielecki, Filipe R.N.C. Maia, Kerstin Mühlig, M. Marvin Seibert, Max F. Hantke, Carl Nettelblad, W. Henry Benner, Martin Svenda, Nicuşor Tîmneanu, Tomas Ekeberg, N. Duane Loh, Alberto Pietrini, Alessandro Zani, Asawari D. Rath, Daniel Westphal, Richard Kirian, Salah Awel, Max O. WiedornGijs Van Der Schot, Gunilla H. Carlsson, Dirk Hasse, Jonas A. Sellberg, Anton Barty, Jakob Andreasson, Sébastien Boutet, Garth Williams, Jason Koglin, Inger Andersson, Janos Hajdu, Daniel S.D. Larsson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 14;nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼30014;nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012 photons per μm2 per pulse. The full-width of the focus at half-maximum was estimated to be 500 14;nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 14;nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.

Original languageEnglish (US)
Pages (from-to)251-262
Number of pages12
JournalIUCrJ
Volume4
DOIs
StatePublished - May 1 2017

Fingerprint

X-Rays
Imaging techniques
X rays
pulses
x rays
Impurities
X ray lasers
contaminants
Wavefronts
Photons
Viruses
Rivers
Virion
Diffraction patterns
Light sources
Evaporation
Lasers
Diffraction
coherent light
viruses

Keywords

  • diffraction before destruction
  • flash X-ray imaging
  • free-electron laser
  • Omono River virus
  • OmRV
  • virus
  • X-ray diffraction

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Daurer, B. J., Okamoto, K., Bielecki, J., Maia, F. R. N. C., Mühlig, K., Seibert, M. M., ... Larsson, D. S. D. (2017). Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. IUCrJ, 4, 251-262. https://doi.org/10.1107/S2052252517003591

Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. / Daurer, Benedikt J.; Okamoto, Kenta; Bielecki, Johan; Maia, Filipe R.N.C.; Mühlig, Kerstin; Seibert, M. Marvin; Hantke, Max F.; Nettelblad, Carl; Benner, W. Henry; Svenda, Martin; Tîmneanu, Nicuşor; Ekeberg, Tomas; Loh, N. Duane; Pietrini, Alberto; Zani, Alessandro; Rath, Asawari D.; Westphal, Daniel; Kirian, Richard; Awel, Salah; Wiedorn, Max O.; Van Der Schot, Gijs; Carlsson, Gunilla H.; Hasse, Dirk; Sellberg, Jonas A.; Barty, Anton; Andreasson, Jakob; Boutet, Sébastien; Williams, Garth; Koglin, Jason; Andersson, Inger; Hajdu, Janos; Larsson, Daniel S.D.

In: IUCrJ, Vol. 4, 01.05.2017, p. 251-262.

Research output: Contribution to journalArticle

Daurer, BJ, Okamoto, K, Bielecki, J, Maia, FRNC, Mühlig, K, Seibert, MM, Hantke, MF, Nettelblad, C, Benner, WH, Svenda, M, Tîmneanu, N, Ekeberg, T, Loh, ND, Pietrini, A, Zani, A, Rath, AD, Westphal, D, Kirian, R, Awel, S, Wiedorn, MO, Van Der Schot, G, Carlsson, GH, Hasse, D, Sellberg, JA, Barty, A, Andreasson, J, Boutet, S, Williams, G, Koglin, J, Andersson, I, Hajdu, J & Larsson, DSD 2017, 'Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses', IUCrJ, vol. 4, pp. 251-262. https://doi.org/10.1107/S2052252517003591
Daurer BJ, Okamoto K, Bielecki J, Maia FRNC, Mühlig K, Seibert MM et al. Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. IUCrJ. 2017 May 1;4:251-262. https://doi.org/10.1107/S2052252517003591
Daurer, Benedikt J. ; Okamoto, Kenta ; Bielecki, Johan ; Maia, Filipe R.N.C. ; Mühlig, Kerstin ; Seibert, M. Marvin ; Hantke, Max F. ; Nettelblad, Carl ; Benner, W. Henry ; Svenda, Martin ; Tîmneanu, Nicuşor ; Ekeberg, Tomas ; Loh, N. Duane ; Pietrini, Alberto ; Zani, Alessandro ; Rath, Asawari D. ; Westphal, Daniel ; Kirian, Richard ; Awel, Salah ; Wiedorn, Max O. ; Van Der Schot, Gijs ; Carlsson, Gunilla H. ; Hasse, Dirk ; Sellberg, Jonas A. ; Barty, Anton ; Andreasson, Jakob ; Boutet, Sébastien ; Williams, Garth ; Koglin, Jason ; Andersson, Inger ; Hajdu, Janos ; Larsson, Daniel S.D. / Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses. In: IUCrJ. 2017 ; Vol. 4. pp. 251-262.
@article{02ab0d0394dc44a8b23f1e97b52c1c64,
title = "Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses",
abstract = "This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 14;nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼30014;nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012 photons per μm2 per pulse. The full-width of the focus at half-maximum was estimated to be 500 14;nm (assuming 20{\%} beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 14;nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.",
keywords = "diffraction before destruction, flash X-ray imaging, free-electron laser, Omono River virus, OmRV, virus, X-ray diffraction",
author = "Daurer, {Benedikt J.} and Kenta Okamoto and Johan Bielecki and Maia, {Filipe R.N.C.} and Kerstin M{\"u}hlig and Seibert, {M. Marvin} and Hantke, {Max F.} and Carl Nettelblad and Benner, {W. Henry} and Martin Svenda and Nicuşor T{\^i}mneanu and Tomas Ekeberg and Loh, {N. Duane} and Alberto Pietrini and Alessandro Zani and Rath, {Asawari D.} and Daniel Westphal and Richard Kirian and Salah Awel and Wiedorn, {Max O.} and {Van Der Schot}, Gijs and Carlsson, {Gunilla H.} and Dirk Hasse and Sellberg, {Jonas A.} and Anton Barty and Jakob Andreasson and S{\'e}bastien Boutet and Garth Williams and Jason Koglin and Inger Andersson and Janos Hajdu and Larsson, {Daniel S.D.}",
year = "2017",
month = "5",
day = "1",
doi = "10.1107/S2052252517003591",
language = "English (US)",
volume = "4",
pages = "251--262",
journal = "IUCrJ",
issn = "2052-2525",
publisher = "International Union of Crystallography",

}

TY - JOUR

T1 - Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses

AU - Daurer, Benedikt J.

AU - Okamoto, Kenta

AU - Bielecki, Johan

AU - Maia, Filipe R.N.C.

AU - Mühlig, Kerstin

AU - Seibert, M. Marvin

AU - Hantke, Max F.

AU - Nettelblad, Carl

AU - Benner, W. Henry

AU - Svenda, Martin

AU - Tîmneanu, Nicuşor

AU - Ekeberg, Tomas

AU - Loh, N. Duane

AU - Pietrini, Alberto

AU - Zani, Alessandro

AU - Rath, Asawari D.

AU - Westphal, Daniel

AU - Kirian, Richard

AU - Awel, Salah

AU - Wiedorn, Max O.

AU - Van Der Schot, Gijs

AU - Carlsson, Gunilla H.

AU - Hasse, Dirk

AU - Sellberg, Jonas A.

AU - Barty, Anton

AU - Andreasson, Jakob

AU - Boutet, Sébastien

AU - Williams, Garth

AU - Koglin, Jason

AU - Andersson, Inger

AU - Hajdu, Janos

AU - Larsson, Daniel S.D.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 14;nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼30014;nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012 photons per μm2 per pulse. The full-width of the focus at half-maximum was estimated to be 500 14;nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 14;nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.

AB - This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 14;nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼30014;nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 1012 photons per μm2 per pulse. The full-width of the focus at half-maximum was estimated to be 500 14;nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 14;nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.

KW - diffraction before destruction

KW - flash X-ray imaging

KW - free-electron laser

KW - Omono River virus

KW - OmRV

KW - virus

KW - X-ray diffraction

UR - http://www.scopus.com/inward/record.url?scp=85018306872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018306872&partnerID=8YFLogxK

U2 - 10.1107/S2052252517003591

DO - 10.1107/S2052252517003591

M3 - Article

AN - SCOPUS:85018306872

VL - 4

SP - 251

EP - 262

JO - IUCrJ

JF - IUCrJ

SN - 2052-2525

ER -