Abstract

Engineered nanoparticles are increasingly used in consumer products. While the potential of these products hold great promise, it is not known what potential toxic effects these nanomaterials may have on human health. There is a need to develop affordable, systematic, short-term in vitro assays aimed at allowing rapid assessment of potential toxicity. The method reported in this paper describes a system in which the intestinal lining is mimicked (Caco-2 human intestinal cell line) and provides an environment in which quantum dots (QDs), and possibly other nanomaterials, can be applied. Transepithelial electrical resistance (TEER) measurements assessed whether the epithelial integrity was breached because of QD exposure. QDs were suspended in calcium/magnesium-free phosphate buffered saline to study non-aggregated QDs. To maintain cell integrity, normal cell culture conditions were retained below the epithelium to provide necessary nutrients and ions. Toxicity studies completed here show that the nanosized QDs coated with hydrophilic thioglycolate capping ligands purchased for these experiments caused disruption in the epithelium monolayer and cell death at 0.1 mg/L of QDs. This toxicity was caused by the nano-size of the QDs rather than the cadmium ions or the sodium thioglycolate capping ligands. Aggregated QDs did not cause toxicity as measured by TEER.

Original languageEnglish (US)
Pages (from-to)955-962
Number of pages8
JournalToxicology in Vitro
Volume23
Issue number5
DOIs
StatePublished - Aug 2009

Fingerprint

Quantum Dots
Semiconductor quantum dots
Toxicity
Assays
Acoustic impedance
Nanostructures
Electric Impedance
Nanostructured materials
Epithelium
Ions
Ligands
Thioglycolates
Consumer products
In Vitro Techniques
Poisons
Cell death
Cadmium
Linings
Cell culture
Nanoparticles

Keywords

  • Aggregation
  • Nanomaterials
  • Quantum dots
  • Toxicity

ASJC Scopus subject areas

  • Toxicology

Cite this

Experimental approach for an in vitro toxicity assay with non-aggregated quantum dots. / Koeneman, Brian A.; Zhang, Yang; Hristovski, Kiril; Westerhoff, Paul; Chen, Yongsheng; Crittenden, John C.; Capco, David.

In: Toxicology in Vitro, Vol. 23, No. 5, 08.2009, p. 955-962.

Research output: Contribution to journalArticle

Koeneman, Brian A. ; Zhang, Yang ; Hristovski, Kiril ; Westerhoff, Paul ; Chen, Yongsheng ; Crittenden, John C. ; Capco, David. / Experimental approach for an in vitro toxicity assay with non-aggregated quantum dots. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 5. pp. 955-962.
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