Excitation dynamics in Photosystem i from Chlamydomonas reinhardtii. Comparative studies of isolated complexes and whole cells

Wojciech Giera, Sebastian Szewczyk, Michael D. McConnell, Joris Snellenburg, Kevin Redding, Rienk Van Grondelle, Krzysztof Gibasiewicz

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Identical time-resolved fluorescence measurements with ∼ 3.5-ps resolution were performed for three types of PSI preparations from the green alga, Chlamydomonas reinhardtii: isolated PSI cores, isolated PSI-LHCI complexes and PSI-LHCI complexes in whole living cells. Fluorescence decay in these types of PSI preparations has been previously investigated but never under the same experimental conditions. As a result we present consistent picture of excitation dynamics in algal PSI. Temporal evolution of fluorescence spectra can be generally described by three decay components with similar lifetimes in all samples (6-8 ps, 25-30 ps, 166-314 ps). In the PSI cores, the fluorescence decay is dominated by the two fastest components (∼ 90%), which can be assigned to excitation energy trapping in the reaction center by reversible primary charge separation. Excitation dynamics in the PSI-LHCI preparations is more complex because of the energy transfer between the LHCI antenna system and the core. The average trapping time of excitations created in the well coupled LHCI antenna system is about 12-15 ps longer than excitations formed in the PSI core antenna. Excitation dynamics in PSI-LHCI complexes in whole living cells is very similar to that observed in isolated complexes. Our data support the view that chlorophylls responsible for the long-wavelength emission are located mostly in LHCI. We also compared in detail our results with the literature data obtained for plant PSI.

    Original languageEnglish (US)
    Pages (from-to)1756-1768
    Number of pages13
    JournalBiochimica et Biophysica Acta - Bioenergetics
    Volume1837
    Issue number10
    DOIs
    StatePublished - 2014

    Fingerprint

    Chlamydomonas reinhardtii
    Fluorescence
    Antennas
    Cells
    Chlorophyta
    Excitation energy
    Energy Transfer
    Chlorophyll
    Algae
    Energy transfer
    Wavelength

    Keywords

    • Chlamydomonas reinhardtii
    • Light harvesting complex I
    • Photosystem I
    • Red chlorophylls
    • Streak camera
    • Time-resolved fluorescence

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology

    Cite this

    Excitation dynamics in Photosystem i from Chlamydomonas reinhardtii. Comparative studies of isolated complexes and whole cells. / Giera, Wojciech; Szewczyk, Sebastian; McConnell, Michael D.; Snellenburg, Joris; Redding, Kevin; Van Grondelle, Rienk; Gibasiewicz, Krzysztof.

    In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1837, No. 10, 2014, p. 1756-1768.

    Research output: Contribution to journalArticle

    Giera, Wojciech ; Szewczyk, Sebastian ; McConnell, Michael D. ; Snellenburg, Joris ; Redding, Kevin ; Van Grondelle, Rienk ; Gibasiewicz, Krzysztof. / Excitation dynamics in Photosystem i from Chlamydomonas reinhardtii. Comparative studies of isolated complexes and whole cells. In: Biochimica et Biophysica Acta - Bioenergetics. 2014 ; Vol. 1837, No. 10. pp. 1756-1768.
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    AU - Redding, Kevin

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    AB - Identical time-resolved fluorescence measurements with ∼ 3.5-ps resolution were performed for three types of PSI preparations from the green alga, Chlamydomonas reinhardtii: isolated PSI cores, isolated PSI-LHCI complexes and PSI-LHCI complexes in whole living cells. Fluorescence decay in these types of PSI preparations has been previously investigated but never under the same experimental conditions. As a result we present consistent picture of excitation dynamics in algal PSI. Temporal evolution of fluorescence spectra can be generally described by three decay components with similar lifetimes in all samples (6-8 ps, 25-30 ps, 166-314 ps). In the PSI cores, the fluorescence decay is dominated by the two fastest components (∼ 90%), which can be assigned to excitation energy trapping in the reaction center by reversible primary charge separation. Excitation dynamics in the PSI-LHCI preparations is more complex because of the energy transfer between the LHCI antenna system and the core. The average trapping time of excitations created in the well coupled LHCI antenna system is about 12-15 ps longer than excitations formed in the PSI core antenna. Excitation dynamics in PSI-LHCI complexes in whole living cells is very similar to that observed in isolated complexes. Our data support the view that chlorophylls responsible for the long-wavelength emission are located mostly in LHCI. We also compared in detail our results with the literature data obtained for plant PSI.

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