Examination of the electrophoretic behavior of liposomes

Michele D. Pysher, Mark Hayes

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The electromigration of liposomes is a complex process resulting in many unexpected behaviors that are difficult to address with existing theories. In this study, the electrophoretic behaviors of liposome populations under various conditions were examined through the use of capillary electrophoresis and the results compared to classical electrokinetic, colloid, and spheroid theories. To elucidate the possible effects of applied field strength, bilayer rigidity, and surface charge on these behaviors, the electrophoretic mobilities of liposome populations were monitored while varying the applied potential, ionic strength of the medium, and the surface charge and cholesterol content of the liposomes. On the basis of comparisons made to the theoretical predictions, our results suggest that liposomal deformation and field-induced polarization may occur during electrophoresis and these mechanisms help to describe many of the observed behaviors.

Original languageEnglish (US)
Pages (from-to)4369-4375
Number of pages7
JournalLangmuir
Volume20
Issue number11
DOIs
StatePublished - May 25 2004

Fingerprint

Liposomes
examination
Surface charge
electrophoresis
Capillary electrophoresis
Electrophoretic mobility
Electromigration
Cholesterol
electromigration
electrokinetics
spheroids
cholesterol
Colloids
Ionic strength
Electrophoresis
rigidity
Rigidity
colloids
field strength
Polarization

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Examination of the electrophoretic behavior of liposomes. / Pysher, Michele D.; Hayes, Mark.

In: Langmuir, Vol. 20, No. 11, 25.05.2004, p. 4369-4375.

Research output: Contribution to journalArticle

Pysher, Michele D. ; Hayes, Mark. / Examination of the electrophoretic behavior of liposomes. In: Langmuir. 2004 ; Vol. 20, No. 11. pp. 4369-4375.
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