Abstract
Laboratory studies of bacterial gene expression have not allowed the investigation of in vivo factors crucial to pathogenesis. Recently, genetic tools have been used to identify in vivo-activated genes. These tools, however, have not allowed the measurement of specific expression levels of virulence-associated bacterial genes in vivo, This report describes a system, previously tested utilizing the fructosyltransferase (ftf) gene of Streptococcus mutans, that allows the measurement of activity of specific virulence-associated streptococcal genes while bacteria are living in the animal environment.
Original language | English (US) |
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Pages (from-to) | 137-142 |
Number of pages | 6 |
Journal | Methods in Cell Science |
Volume | 20 |
Issue number | 1-4 |
DOIs | |
State | Published - 1998 |
Externally published | Yes |
Keywords
- Gene expression
- Mammalian environment
- Streptococcus
ASJC Scopus subject areas
- Cell Biology