Examination of streptococcal gene expression in the mammalian environment

W. Todd Grey, Joshua D. Lasker, Roy Curtiss, Michael C. Hudson

Research output: Contribution to journalArticle

Abstract

Laboratory studies of bacterial gene expression have not allowed the investigation of in vivo factors crucial to pathogenesis. Recently, genetic tools have been used to identify in vivo-activated genes. These tools, however, have not allowed the measurement of specific expression levels of virulence-associated bacterial genes in vivo, This report describes a system, previously tested utilizing the fructosyltransferase (ftf) gene of Streptococcus mutans, that allows the measurement of activity of specific virulence-associated streptococcal genes while bacteria are living in the animal environment.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalMethods in Cell Science
Volume20
Issue number1-4
DOIs
StatePublished - Dec 1 1998

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Keywords

  • Gene expression
  • Mammalian environment
  • Streptococcus

ASJC Scopus subject areas

  • Cell Biology

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